The Dynamic Changes Of Serum MBP And Histological Observation Of The Injured Spinal Cord Of Rats After Microencapsulated Rabbit Schwann Cells Are Transplanted Into Them

Objective To observe the dynamic changes of serum Myelin basic protein (MBP) and histological changes on the injured half transection of adult rat after transplantation of the microencapsulated Schwann cells(SCs), in order to study the mechanism how the transplantation of microencapsulated different race of SCs helps to treat the spinal cord injury(SCI).Method A total of 142 adult Sprague-Dawley(SD) rats were randomly divided into four groups: Group A(n=44), B(n=44), C(n=44) and D(n=10). Group D was the normal control. Group A, B and C were performed a right hemisection cutting of spinal cord at T₁₀ level, then SCs (into Group B) and with microencapsulated SCs (into Group C) were implanted into the relevant injured area respectively. Group C was implanted nothing. 1,3, 7, 14 and 28 days after the injury, 8 rats of each group in A, B and C as well as the normal rats were anesthetized and perfused transcardially with 4% paraformaldehyde(the pH7.4). Took out the thoracic cord near the injured site and the spinal cord of the normal rats at corresponding level, performed the paraffin section. Then dealed with HE staining and Loyez myelin staining. Before animal perfused, samples of blood were obtained from heart by puncture. Then the content changes of MBP were measured by ELISA examination. In addition, 2 and 8 weeks after the injury , 2 rats of each group in A, B, C were perfused and embedded in Epon816, performed uranyl acetate and lead citrate double staining, slices were observed with the electron microscopyo Result On the 1^st day after SCI, the content of serum MBP increased...