Experimental Study Of Inhibitory Effect Of Indomethacin On Laryngeal Cancer Cell Hep-2

Non - steroid anti - inflammatory drug (NSAIDs) is a kind of drugs of de-phlogistic and antipyretic analgesic speciality. In recent epidemiologic studies showed a decline in the risk with common intake of NSAIDs for malignant tumors such as colon, breast, lung, and prostate cancer; Previous studies found NSAIDs inhibits proliferation in all kinds of tumor cells in vitro and in vivo, and it induces cell apoptosis. However the investigations about the effect of NSAIDs on laryngeal cancer little known. The laryngeal cancer is a common squamous cell carcinoma of the head and neck. The presence of regional metastasis in patients of laryngeal cancer is a common and adverse event associated with poor prognosis and high mortality. The incidence rate of this cancer in male is four to twenty times higher than in female. Indomethacin is a represent of NSAIDs. In the present study, we investigated the effect of indomethacin on the proliferation and apoptosis of laryngeal cancer cell lines, Hep - 2 cells, and gained the clear evidence about that indomethacin has depressant effect on them. We report here that exposure of Hep - 2 cells to indomethacin resulted in morphology changes, a dose - dependent and time - dependent growth inhibition, apoptosis, This study provided convincing evidence that indomethacin as the represent of NSAIDs has the strong potential as an intervention for the treatment and prevention of laryngeal cancer.In this study, we fund 1, indomethacin inhibits proliferation of laryngeal cancer line, Hep -2. MTT colorimetric method showed indomethacin significantly suppressed the cell growth with a marked dose - dependent and time - dependent inhibitory effect on Hep - 2 cell growth. Flow cytometric analysis show...