Intervertebral Disc Cell Therapy For Regeneration: Marrow Mesenchymal Stem Cells Cultivation In Vitro And Intervention In TGF-β₁ Implantation In Rabbit Intervertebral Discs

Abstract Background:Spinal disorders are a leading source of disability and morbidity, resulting in substantial pain and increased health care costs for patients,and also as a major socioeconomic impact in morden times. Many of these disease such as are the direct or indirect result of Intervertebral Discs Degenerative (IDD).Current treatment options, including both conservative and surgical measures,target the clinical symptoms of intervertebral disc degeneration,as opposed to addressing the pathologic processes occurring early in the course of degeneration. However, recent molecular biology technology development may allow for the treatments focusing on the cellular changes that occur in this disease.In recent years the study of transplant intervertebral discs or nucleus pulposus to restore degeneratived intervertebral discs have achived great progress.However, it is difficult to draw the nucleus pulposus cell from the body in vitro culture,and also hard to multiplication.So the most significance is to provide high-quailty and quantity cells,it is what we should investigate nowadays.Recent studies of MMSCs that one of adult stem cells have reported successful applications of MMSCs transplantation in various fields. The merit of MMSCs is that can be obtained in local anesthesia,and cultured in vitro, amplificated to enough quantity in short times. They possess the ability to differentiate into varieties of cells found in connective tissues of mesoderm origin.Their ability to differentiate is governed mainly by the environment in which they are placed. Conditions favorable for inducing osteogenesis, chondrogenesis and adipogenesis have been well established. Transplanted the cells that induced by autologous MMSCs to autos for restore can not generate immunological rejection is anther merit of MMSCs.Within advanced study in MMSCs in recent years, the concept of therapeutic-cloning is that combining the ability of located,induced,and differentiated of MMSCs with tissue-engineering is prefered for the direction of investigation in the future[1].MMSCs is the best stem cells using in intervertebral discs tissue-engineering todays.