Study On Component Analysis Of Human Gallstones

With the enhancement of the living standard and the change of the dietetic habit of people, Cholelithiasis is becoming a popular disease. It's indicated that cholelithiasis reaches 11.53% in surgery diseases. Recently, with the appearance of methods to dissolve and discharge gallstones it becomes more and more important to know the components of gallstones, so that we can choose different kinds of drugs and treatments to cure cholelithiasis. Furthermore, studies of the components of gallstones can be the basis of further research to find out its generation , the diagnosis and therapy methods. The gallstones were mainly composed of cholesterol, bilirubin, various cholic acids, proteins, dissociate fatty acids and micro-quantity of metal elements such as Ca, Mg, Na etc. It has been reported that methods of TLC Scanning, Spectrophotometry and FTIR had been employed to assay the component of gallstones. However, all of them were used to determine only a single component, they are lack of systematic and inconvenient to use.This thesis described the study of the qualification and quantification research of 16 human gallstones. The contents are listed out as following:1. An HPLC method with ELSD and UV detector on-line was established to determine cholesterol, bilirubin and various cholic acids in gallstones simultaneously.2. FTIR- ATR was used for the qualification and classification of Gallstones, and also employed to quantitatively determine cholesterol and bilirubin in human gallstones simultaneously. A comparison of the assay results between HPLC method and FTIR-ATR method was done.3. The contents of micro-quantity of metal elements were determined by ICP-MS in human gallstones.Above the three methods have the advantages of reliableness, efficiency and convenience. They are expected to benefit the diagnosis and treatment of Cholelithiasis.