| Objective To investigate the effects of morphous and function onfetal brains in pregnant rat of high cholic acid. To explore the differenceof the serum level of neuron-specific enolase (NSE) in differentconcentration of cholic acid. And to study the correlation betweenneuron-specific enolase and cholic acid.Methods Randomly deviding 30 SD pregnant rats to three groupsA,B and C, every group is 10. From 13th to 20th duration of pregancy,injecting 5.5mg·kg-1-d-1 cholic acid to pregnant rats of group A,1.4mg·kg-1·d-1 cholic acid to group B and the partes aequales normalsaline to group C by intraperitoneal injection one time every day. In the21th duration of pregancy, to cut the belly open and take the fetus out andrecord the total fetus, live fetus and the weight. Determine the serumconcentration of total bile acid (TBA) in pregnant rats and fetal rats.Enzyme linked immunosorbent assay (ELISA) was used to detect theserum level of neuron-specific enolase (NSE) in fetal rats. Fix and embedthe brain after decapitation, then to observe the pathological change of thefetal cerebrum under light microscope and electron microscope.Result1. Before intervention, there is no significant difference of the serumconcentration of the total bile acid(TBA), alanine aminotransferase (ALT),asparatate aminotransferase(AST), tatal bilirubin(TBIL) and direct bilirub (DBIL) of pregnante rats in groups A, B and C; P＞0.05. Afterintervention, there is also no significant defference of the serum. Theserum concentration of TBA, ALT, AST, TBIL, DBIL of pregnante ratsin group A was significantly higher than that of group C, P＜0.05. Butthere is no significant difference between group A, C and B, both areP＞0.05. There is significant defference of the serum concentration ofTBA of pregnante rats in every two groups, P＜0.01.2. The serum concentration of TBA of pregnant rats and fetal rots ingroup A is (22.32±8.12)μmol/L and (28.84±8.06)μmol/L, in group B is(9.77±3.56)μmol/L and (9.34±3.55)μmol/L, in group C is(3.60±1.78)μmol/L and (3.95±1.19)μmol/L. There is significantdefference in every two groups, P＜0.01. The serum concentration of TBAbetween pregnant rats and fetal rats were positively correlated with eachother, r=0.875.3. The mortinatality of fetus in group A, B and C are 30.10%,16.85% and 7.06%, there is significant defference in every twogroups, P＜0.05. The average weight of fetus in group A wassignificantly lower than that of group B and C, [(4.24±0.98)g vs(5.82±0.32)g and (6.14±0.77)g, both are P＜0.05]. But there is nosignificant difference between group B and C, P＞0.05.4. The serum lever of NSE of fetal rat in group A was significantlyhigher than that of group B and C, [(31.90±13.12) ng/ml vs (13.61±5.97) ng/ml and (9.32±3.94)ng/ml, both are P＜0.05]. But there is no significantdifference between group B and C, P＞0.05. The serum level of TBA andNSE in fetal rat were positively correlated with each other, r=0.758.5. Under the light microscope we found that the neuronaldegeneration and necrosis. The lever of organization disorder, the densityof nerve cells decrease and the cell nucleus pyhnosis and anachromasis.The neuronal degeneration area in group A and B are significantly higherthan group C, [(1.38±0.58) and (1.48±0.66) vs (0.72±0.30), both areP＜0.05]. But there is no significant difference between group A and B,P＞0.05. The is no apparente correlation between the neuronaldegeneration area and the serum level of NSE in fetus, r=0.282. Theneuronal necrosis area in group A are significantly higher than acid groupB and C, [(1.84±0.73) vs (0.86±0.38) and (0.58±0.27), both are P＜0.05].But there is no significant defference between group B and C, P＞0.05.The neuronal necrosis area and the level of NSE in fetus were positivelycorrelated with each other, r=0.798, neuronal degeneration and necrosis.The level of organization disorder, the denisty of nerve cells decrease andthe cell nucleus pyknosis and anachromasis.6. Under the electron microscope we found that the neuronalnuclear membrance ambiguity, karyopycnosis, nucleolus disappeared,nuclear chromatin rarefaction in the parietal lobe of fetal rats' cerebralcortex. The number of endoplasmic reticulum and mitochondria decrease, the residual mitochondria swelling, cristae quassation and disappearing.Under the electron microscope 25000 magnification, the number ofmitochondria of fetal rats'cerebral cortex in group A is significantlylower than that of group B and C, [(21.92±9.00)μm-3vs(45.48±13.12)μm-3 and (36.13±12.13)μm-3, both are P＜0.01]. But thereis no significant difference between group B and C,P＞0.05. The volumeof mitochondria of fetal rats'cerebral cortex in group A and B issignificantly higher than that of group C, [(7.01±1.79)×10-4μm3 and(5.66±1.57)×10-4μm3 vs (3.15±1.21)×10-4μm3, both are P＜0.01]. Butthere is no significant difference between group A and B,P＞0.05.Conclusion There is apparente pathological change of fetal ratsbrain in cholic acid groups, the neuronal degeneration and themitochondria swelling was mainly found in low cholic acid group, theneuronal necrosis and the mitochondria decrease was mainly found inhigh cholic acid group. The serum concentration of TBA and NSE in fetalrats were positively correlated with each other.
|
PDF Full Text Request