The Effect And Mechanism Of Sophoridine And Oxysophoridine On Central Nervous System

1. Research on central pharmacological effect and mechanism of oxysophoride. Objective To study the central pharmacological effect of oxysophoride and its basic mechanism.Method Behavior pharmacological method was adopted to observe and monitor the effect of oxysophoride on mice's general behavior and autonomic activities, on the action of hypnotic of Pentobarbital with threshold and subthreshold dosage and on mice's passive movememt as well as the effect on Pentylenetrazol induced convulsions in mice. Monopole leads were employed to record the bioelectricity of cerebral frontal cortex, amygdale, hippocampus, and reticular formation relatively. FFT technique was adopted to analyze the cerebral electrical waves for revealing the effect of oxysophoride on cerebral bioelectricity of different parts of brain in rats. SABC was introduced to measure the effect of oxysophoride on glutamate(Glu) and gamma amino butyric acid(GABA)immunoreaction positive cells of frontal cortex and hippocampus in CNS(central nerves system).Results Oxysophoride administrated by ip(intraperitoneum injection ) at 250mg/kg,500mg/kg and 1000mg/kg suppressed the autonomic activities at the rate of 42.6%,57.2%and75.3% respectively, shortened the time of falling asleep induced by Pentobarbital at the rate of 38.0%,49.0% and 39.7%,prolonged the sleeping time by 172%,138%and 389%,enhanced the hypnotic effect of Pentobarbital at subthreshold dosage.Our study shows no effect on passitive moment and convulsion caused by Penlylenetrazol by ip administrating Oxysophoride1000mg/kg.