Isolation And Characterization Of Anti-Candida Albicans Chemical Components From Chinese Traditional Herb Alpinia Officinarum Hance

Alpiniae officinarum Hance is one of important medicinal plants growing mainly in Southern China. Pharmacological experiments indicate that this plant contain chemical components showing anti-ulcer, anti-diarrhea, antalgic effect, anti-inflammatory, hypoglycaemic activity, antioxidant effect, and antibiotic functions. Our results show that the acetoacetate and chloroform extractions revealed different anti-Candida albicans activities. In order to characterize the antifungal components, using column chromatography approaches, including Silica gel, Toyopearl HW-40 and preparative HPLC, we isolated and purified 6 antifungal compounds from the chloroform extraction. The structures of the 6 compounds were identified by means of 1H-NMR,13C-NMR and 2D-NMR spectra. Among the 6 compounds, there are three diarylheptanoids, two flavonoids and one diterpene, their structure were 7- (4-hydroxyphenyl)-1-phenylhept-4-en-3-one (1), 5-hydroxyl-1-(4-hydroxyphenyl)-7- phenylheptan-3-one (2), 1-(4-hydroxy-3-methoxyphenyl)-7-(4-hydroxyphenyl) heptane-3,5-diol (3), galangin (4), apigenin (5), and 16-oxo-8(17),12-labdadien-15- oic acid (6). Compound 3 and 6 were isolated for the first time from this plant.All isolated compounds were screened by antifungal bioassay and showed bioactivities of different degrees. Compound 1 exhibited the most significant anti-Candida albicans activity with 57.1% antifungal potency and MIC 0.1~1mg/mL. The MIC of Compound 2~6 are 0.01~0.1mg/mL, 0.01~0.1mg/mL, 1~2mg/mL, 0.1~1mg/mL, 0.1~1mg/mL, while their antifungal potency are 44.8%, 27.8%, 54.5%, 44.1%, 48.5%.