Expression And Significance Of TSG101, MDM2 And ER In Benign And Malignant Ductal Hyperplasia Lesions Of The Breast

Objective To investigate the correlation of tumor susceptibility gene 101 (TSG101), murine double minute-2 (MDM2) and estrogen receptor (ER) expression with tumorgenesis of breast carcinoma.Methods Formalin-fixed, paraffin-embedded tissues of 30 cases of breast usual ductal hyperplasia (UDH), 30 cases of breast atypical ductal hyperplasia (ADH) and 36 cases of breast infiltrating ductal carcinoma (IDC) were detected by immunohistochemistry assay using monoclonal antibodies of TSG101, MDM2 and ER. The results were analyzed by statistical software SPSS10.0.Results 1. The expression of TSG101 protein was positively detected in 66.7% (20/30) UDH, 56.7% (17/30) ADH and 36.1% (13/36) IDC, respectively. There presented a statistic difference between UDH and IDC groups (P<0.05) for the positive rate and there was a negative correlation between TSG101 expression in IDC and lymph node metastasis of this cancer (P<0.01). 2. The expression of MDM2 protein was positively detected in 30.0% (9/30) UDH, 56.7% (17/30) ADH and 75.0% (27/36) IDC, showing distinct difference between UDH and ADH groups (P<0.05), UDH and IDC groups (P<0.01) respectively. 3. The expression of ER protein was positively detected in 36.7% (11/30) UDH, 60.0% (18/30) ADH and 63.9% (23/36) IDC respectively, showing a significant difference between UDH and IDC groups (P<0.05) and there was a statistic significant difference between UDH and moderate to high grade ADH groups (P<0.01) as well. 4. The expression of TSG101 was negatively related to the expression of MDM2 and ER during tumorgenesis of breast carcinoma. In addition, a positive correlation between MDM2 and ER expression in the course was found.