| Human paillomavirus (HPV) infection causes virtually all cases of cervical cancer, the second most common cause of death from cancer among women worldwide, and external genital warts, threatening the health of human race severely.A complete life cycle of HPV depends on the differentiation process of epithelial cells, precluding proliferation of infectious virus particles from conventional cell culture, and only very limited virus particles can be extracted from patients'tissues, which influenced the HPV research and evaluation of protection efficiency of HPV candidate vaccines. A variety of systems have been developed to overcome the limitation of the virus. Several types of infectious papillomaviruses can be obtained by human xenograft or organotypic raft culture, and pseodoviruses can be constructed using recombinant Semliki Forest virus (SFV), vaccinia virus and yeast expression systems. But all these systems are time-consuming, technically demanding and relatively low-yield.Based on the advances in the field of HPV study and the condition of our lab, plasmids containing the codon optimized HPV structural genes and reporter plasmid were co-transfected, successfully generating high titer infectious pseudoviruses of HPV16, HPV18, HPV6 and HPV11. Electron micrograph identified that the pseudoviruses we obtained were morphologically similar with the intact HPV, and subsequently, using control monoclonal antibodies, the pseudoviruses were found to infect mammalian cells like intact HPV, suggesting that the pseudoviruses could be used to study the molecular biology of HPV and evaluate the protection efficiency of HPV candidate vaccines.The 4 types of HPV pseudoviruses were utilized to study the sensitivity of 29 mammalian cell lines derived from different tissues and species to HPV infection. HPV16 and HPV18 pseudoviruses could infect various cell lines derived from human, rodent, monkey and rabbit, while HPV6 and HPV11 pseudoviruses could hardly...
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