Fabrication Of Polymerized Crystalline Colloidal Arrays And Their Sensing Applications

The detection sensitivity of biomolecules depends on the quantity and density of the immobilized biomolecules on the substrate. Enzyme linked immunosorbant assay (ELISA) always uses polystyrene microplates as the substrate. The research in recent years shows that the biomolecule adsorptivity of the substrates like microplates are limited by their restricted surface area and fabrication techniques. In this dissertation, some porous materials with larger surface area and better biomolecule adsorptivity were fabricated via different methods to improve the sensitivity of RIFS detection, which could provide real-time, in situ and dynamic detection. We also compared the biomolecule adsorptivity of these porous materials with flat polystyrene materials.The main contents of this dissertation are as follows:1. We fabricated Polystyrene (PS)-Silica (SiO2) porous layers on the microscope slides via spin-coating method. A lab-made reflectometry interference spectroscopy (RIFS) setup was used to observe the binding action of BSA on the porous substrates.2. We fabricated ordered SiO2 (100, 200, and 300 nm) porous materials with structural color and discussed the reason of formation of the structural color.3. We fabricated polystyrene macroporous materials using SiO2 porous materials as templates. RIFS was used to observe the binding action of BSA on the materials.