Effects Of Dispersal Liver Dissipate Blood Stasis Tongluo Jiang Zhuo Decoction On Expression Of Intrahepatic MD2,iNOS In Mice With Nonalcoholic Hepatic Steatosis

Background And Objective: With the improvement of the people's living level,the morbidity of fatty liver is higher and higher and it becomes the second liver disease in some area .Besides aicoholic fatty liver because of kissing the cup, the incidence of Nonalcoholic fatty liver disease(NAFLD) rises daily.Of late years ,after studying NAFLD's result we find 50 percent of NAFLD advances to liver fibrosis and 15 percent advances to hepatocirrhosis,and 3 percent advances to crock up or needing liver transplantation .15 persent to50 percent of NAFLD sufferers have appear liver fibrosis or hepatocirrhosis when they go to see a doctor,which makes the scholars of liver disease attach importence the disease and study it deeply.After analyzing ninety NAFLD suffers Willner etc.fund that more and half of them go with diabetes heperlipemia ,hypertension ,fatness and most of them have limosis insulin level rise,so they think NAFLD is behavior of metabolize synthesis (MS) or insulin insistence synthesis in liver .The more serious end is that it's prognosis isbad , 28 percent NAFLD sufferers have hepatocirrhosis ,and half of them need liver transplantation because of portal hypertension.Today,we have no effective method for NAFLD because of we are lack of knowledge on it's pathogensis . In this experiment,I make mice have NAFLD by feeding fat diet ,and treat it by using of dispersal liver dissipate blood stasis tongluo jiangzhuo decoction to observe Triglyceride (TG) , totalcholesteral(TC) ,beta-lipoprotein(LDL),high-density lipoprotein(HDL) in serum,and MD2,iNOS in liver tissue,so we can find possible echanism ofaction of the Chinese medicine.Method: Sixty Spague-Dawlay(SD) mice were randomly divided into seven groups, which were named common group(C)(n=10),model group(M)(n=10),low dosage treat group(LT)(n=8),modest dosage treat group(MT)(n=8),high dosage treat group(HT)(n=8),preventing group (P)(n=8)positive comparison group (D)(n=8).The C group mice were feed with normal diet for 12 weeks.The other groups mice were feed with fat diet which contained 88% common feedingstuff.2%cholesterin and 10% lard for 12 weeks and at the sametime ,filled stomach with fat fluidity which contained 10% cholesterin and 20%lard and 1% cholate natrium and 0.025%tapazole and 20% malonachol and 20% tuwen and distilled watter for 12 weeks ,and drunk. water freedom.The mice in LTgroup MT group and HT group were filled stomach with decoction at 0.234mg/kg.d,1.17mg/kg.d,5.85mg/kg.d concentration when at the beginning of the fifth weeks.The mice in D group were filled stomach with 0.0009%liputo at the beginning of the fifth weeks.The mice in P group were filled stomach with 1.17mg/kg.d decoction.Kill all the mice at the end of 12 weeks,with method of make the mice fasting last night,and morrow weigh them.Anesthesia with diethyl ether and collected blood,weigh the liver,store a part of the liver. The pathological changes were observed with HE staining and Sudanâ…¢staining, the amount of serum TG,TC, LDL, HDL,were measured and MD2,iNOS in liver tissue were also measured by using of RT-PCR method.Results:1,General observing:The mice in C group were agile and activity,but in M group were contrary.The liver in C group was bright red and elastic,but in M group was suff color and tough and tensile,in LT,MT,HT groups were medial.2,The changes in liver tissue after 4 weeks in M group compare with C group were fatty in evidence,and hepatic lobule structure disappeared : After 12 weeks compar with M group , liver tissue of the mice in MT group appear fat and inflammation slighter3,The changes in blood serum : After 4 weeks ,TC,TG,ALT,LDL,in LT,MT,HT,M,P,D groups comparing with C group all increased,butHDL decreased (P<0.05). After 12 weeks,the consequence of biochemical docimasia show that comparing with M group ,the level of TC decreased in other groups (P<0.01).The level of TG decreased in all groups,and in MT,D group in evidence (P<0.05).The level of ALT decreased in all groups and in MT group in evidence (P<0.05).The level of LDL decreased in all groups (P<0.05),but HT group.The level of HDL increase in MT and p groups in evidence (P<0.01).4,The presentation of MD2mRNA and iNOSmRNA in liver tissue : After 12 weeks,the consequence of biochemical docimasia of MD2 mRNA in LT,MT,D,P group decreased compare with M group ,but MT group decreased obviously.The level of iNOSmRNA was low in LT,MT,D,P groups compare with M group (P<0.01),and MT group correspond with C group.Conclusion: MD2,iNOS are take part in the with the Pathologysiology course of NAFLD ; The Chinese Herbs can cure and prevent the further development of NAFLD. The MT groups were obviously.