Expression Of Transforming Growth Factor-β₁ In Human Lens Epithelial Cells With Different Stage Of DRP

Objective: To investigate the expression of transforming growth factor-β1 (TGF-β1) in human lens epithelial cells(LECs) from cataract patients with or without type 2 diabetes mellitus. To study the possible effects and mechanisms of TGF-β1 on type 2 diabetes patients with cataract.Methods: LECs attached to the anterior capsules of cataract patients with the clinical diagnosis of type 2 diabetes mellitus (diabetic groups,75 cases)and without diabetes mellitus (control group,20 cases) were collected to determine the protein expression of TGF-β1 by Western blot analysis.Results:(1)Independent sample T test: It was proved that the mean of TGF-β1/β-actin was lower in the control group(0.819±0.122)than that in the diabetic groups(1.936±0.762)(P<0.05),this result implies that the TGF-β1 protein's expression level was lower in the control group. (2)One-way ANOVA: homogeneity of variance checking showed F=7.491,P=0.00 heterogeneity of variance was compared and contrasted by using Tamhane's T2, the result showed that①there was no statistic differences of the TGF-β1 protein's expression level in LECs between control group and DRP ( diabetic retinopathy ) 1 stage group(P=0.22),but there were significant differences among other diabetic subgroups(P<0.05).②for the diabetic groups, there was significant difference between DRP1 stage group and each of the other subgroups(P<0.05);the difference between DRP2 stage group and DRP5 stage group was much more significant(P<0.01); the rest of the DRP subgroups are more or less similar(P>0.05).(3)diabetic subgroups simple correlation analysis: the non-parametric Spearman correlation analysis showedthat the TGF-β1 level in the corresponding Type 2 diabetic cataract lens epithelial cells tended to increased with the diabetic retinopathy progressing.Conclusion: TGF-β1 may play an important role in the development of cataract in type 2 diabetes patients. The progress of diabetic retinopathy is correlated to the TGF-β1 level in cataract lens epithelial cells of the corresponding type 2diabets patients.