The Expression And Clinic Significance Of Guanylyl Cyclase C MRNA In Peripheral Blood Of Colorectal Cancer Patients By RT-polymerase Chain Reaction

Objective: To investigate the expression of GC-C in peripheral blood of colorectal cancer patients and to discuss it's clinical significance.Methods: Use the RT- polymerase chain reaction to detect the GC-C mRNA in peripheral blood of colorectal cancer patients and analysis them with clinical and pathological indexes statistically.Results: The positive rates of GC-CmRNA in 52 cases were 48.08%(25/52), The positive rates of GC-C mRNA were associated with infiltration (P<0.05) and tumour embolus in vessel (P<0.05), the differences were significant . While to CEA level in peripheral blood (P>0.05). there were no statistical difference. The positive expression of GC-CmRNA increased with the clinical stage . In the 29 cases with normal CEA level, we found 9 cases with GC-CmRNA expression, which including 4 cases with tumour embolus in vessel and 2 cases with cancer tubercle . While in 26 cases with no GC-CmRNA expression we found 6 cases with high CEA level, which including 2 cases with tumour embolus in vessel.Conclusion: Guanylyl cyclase-C expressed selectively in cells derived from intestinal mucosa, including normal intestinal cells , primary and metastatic colorectal cancer cells , but not by extragastrointestinal tissues and tumors. Guanylyl cyclase-C was the valuable new clinical index in micrometastasis diagnosis, stage and postoperation of colorectal cancer.