Expression And Clinical Significance Of GC-C Gene In Peripheral Blood Of Patients With Colorectal Carcinoma

Objective To investigate the expression and clinical significance of Guanylyl cyclases C( GC-C ) gene in peripheral blood of patients with colorectal cancer. Methods Using guanylyl cyclases C mRNA (GC-C mRNA) as a target gene, GC-C mRNA had been examined by reverse transcriptase polymerase chain reaction (RT-PCR ) in ten colorectal carcinoma tissues and peripheral blood of forty-eight colorectal cancer patients,twenty healthy controls,fifteen benign colorectal diseases patients. Results GC-C expression was showed in ten colorectal carcinoma tissues.The expression rate of GC-C gene was 11.1%(1/9),29.4%(5/17),66.7%(8/12),90.0%(9/10)in patients with colorectal cancer of Dukes stage A,B,C,D respectively(P<0.05),but none both in twenty normal controls and fifteen benign colorectal diseases patients' blood samples. Conclusions colorectal cancer might be regarded as systemic disease even in early stage. The expression rate of GC-C gene in peripheral blood has a highly correlation with the Dukes stage. No significant difference was found about the expression of GC-C gene among primary tumor site,patient's sex and age.Detecting the expression of GC-C in peripheral blood of colorectal cancer patients may contribute to improve clinical staging and indications for therapy .