| Background and Objective: Root Canal Therapy is the basic method of treating endodontic and periapical diseases in clinic.The general trend of Root Canal Therapy shown in three aspects: Try to design and manufacture better expanding appliances is the first, secondly, develop and compound more powerful antiseptic. Thirdly, try to work out a special type of fillings to better seal the root canal, which has the better disinfection function as well. Because of this, fillings to root canal is the main study direction toward the root endodontics treatment research. In recent years, the main basis of fillings we normally use which is self-made are zinc oxide, iodoform, thymol and rosin etc. All the factors are mixed with a special processing way to strength the hardness and fluidness as well as plasticity while it being filled. This type of fillings can be pressed when filling with the purpose of better closing to root canal, especially it has been proved a satisfactory clinic result for treating the bending root canal, C type root canal, side pulling root canal as well as various of root canals. Because of the lower cost as well as the easily operated way, the successful rate can be greatly improved for the root canal therapy with a reduced cost, shorten the treatment time. However, until today, there is no official comprehensive comparison between the closing ability, tolerance ability, anti-bacteria ability and current or future clinic result of the special fillings and the ordinary fillings which is composed of zinc-oxide, clove cataplasm, teeth pastern. In order to obtain the theory foundation for the past root fillings promotion, this study is going to set zinc-oxide, clove cataplasm and teeth pastern as a comparison party, to compare and analyze the two different materials by adapting basic experiments and the clinics. Material and Methods1 : micro-leakage experiment: select 30 fresh single root canal teeth and divide them into 3 groups. Group A: using past root fillings; Group B: using zinc-oxide, clove cataplasm, teeth pastern; group C: no filling but preparation only. Compare the sealing ability by using coloration method, Result and Conclusions: average length of coloration thread of group A is 2. 37 mm, group B of 4.27 nun and group C of 7.56 mm. It has an obvious difference (P<0. 05) based on the statistics analysis for this two different group.2: anti-bacteria experiment: Under anaerobic condition, take Bi bacteria and spread it on BHI blood plate. In the meantime, inoculate Sa bacteria on agar blood plate. Make eight plates for each bacteria and then take a piece of paper of 6mm to dip each of them for 0. 01ml, and then grow them both under oxygen situation and anaerobic situation. Here is the result: the anti-bacteria ability of past root fillings precede the other group. Results and Conclusions:There is an obvious difference (P < 0. 05 ) according to the statistics analysis. Past root fillings has a certain anti-bacteria function both for the Sa and Bi, but there is no obvious difference (p>0.05). However, teeth pastern group only has the anti-bacteria function on Bi with an obvious difference (P<0. 05) . 3: Cell toxicity experiment: Mix MTT with these cells to let them grow for 4 hours, and then use dimethylsulfoxide to dissolve the MTT rime among active cells to get purple liquid, ELISA inspector can be used to measure the light imbibe value, as the light imbibe value has the direct ratio against the quantity of cells which can easily reflect how much cells in total quantity. Please be noticed these cells we adapt is HGF, culture medium is the DMEM. Results and Conclusions: Cell toxicity of both past root fillings and teeth pastern group are bigger than the blank group, however there is no obvious difference ( P >0. 05) between this two groups. 4: Clinic curative effect contrast: To evaluate the short-term clinic curative effect between past root fillings and teeth pastern group. Divide ill teeth into two groups at random: Group A: past root fillings; group B: zinc-oxide, clove cataplasm plus teeth pastern. Periodically check the operation effect after one month, 3 month and 12 month to compare the clinic curative effect between them. Here is the result based on the statistics: The success rate for group A is 92. 5%, 95. 5%, and 88% for the time of one month, 3 months and 12 months; It is 79.51%, 89. 7% and 81. 6% for the same time frame. There is an obvious difference of the clinic curative effect which group A is better than that of group B for the time of one month. Results and conclusions:There is no obvious difference on effect for the time of three month(P > 0. 05). However the operation filling time of group A is shorter than that of group B regardless it is front teeth or back teeth. There is an obvious difference (P<0.01) between them. :... |
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