Study On The Protective Effects Of HuaXian Capsule On Hepatic Fibrosis In Rats

Objective: To study and explore the pathologic machanism of hepatic fibrosis(HF) and to observe the effect of Huaxian(HX) capsule on the effectiveness of anti-hepatic fibrosis, we made this study.Mathods: The rats models of HF were made by the modified and complexed mathod. The rats were randomized into five groups: black control group, model control group, Colchicine(COL) group, low dosage HX(LHX) group and high dosage HX(HHX) group, and all these groups were treated separately with normol solution(NS), Colchicine, low dosage HX and high dosage HX respectively. Then the content of ALT, AST, TBA, LN, HA and PIIINP in rats' serums were detected; the section preparations of morphosis changes of rats' liver tissues were observed under microscope after HE staining.Results: Compared with blank control group,the content of ALT, AST, TBA, LN, HA and Pâ…¢NP in serums of rats of model control group all markedly raised(P<0.01), and morphosis of liver tissues of those were markedly destroyed and changed. Compared with model control group, the content of ALT, AST, TBA, LN, HA and Pâ…¢NP in rats' serums of LHX group and HHX group markedly reduced(P<0.05 or P<0.01). Compared with COL group, the LN content in serums of rats of LHX group markedly reduced(P<0.05). The other indices among the groups of treatment had no significant differences statistically (P>0.05) .Changes of liver tissue morphosis of rats of COL group, LHX group and HHX group all improved differently and those changes of LHX group improved most markedly.Conclusion: Huaxian capsule can markedly reduce the contents of ALT, AST and TBA in serums of the tested rats. It is suggested that the drug has some influences on the protection of liver and decreasing the contents of enzymes; The capsule can significantly reduce the contents of LN, HA and Pâ…¢NP in serums of rats. It is indicated that the drug can improve collagen metabolism,decrease the synthesis and (or) improve degradation of Extracellular Matrix(ECM); The capsule has the different influence on reducing degeneration and necrosis of liver cells, decreasing hyperplasia of collagenous fibres and remodelling microconstruction of liver tissue. It is shown that the drug has the effect on anti-hepatic fibrosis. Compared with COL, the effect of capsule is superior to that of it in some indices. The effect of LHX group is better than that of HHX group, which suggests that LHX capsule should be used to cure hepatic fibrosis.