Isolation Of CD133～+ Cells From Human Renal Cell Carcinoma By Magnetic Cell Sorting System

Objective To evaluate the role of CD133~+ progenitor cells in the development of renal cell carcinoma, cytofluorimetric analysis was used to study renal cell carcinoma, a practical method was established to isolate and purify CD133~+ cells by the immunomagnetic microbeads .Methods Presence of a cell population expressing CD133 marker in cells dissociated from tissue specimens of 30 renal cell carcinomas was investigated by cytofluorimetric analysis.CD133~+ cells were isolated by magnetic cell sorting using the MACS system. Microarray study on CD133~+ cells and CD133~- cells was performed after magnetic isolation.Results CD133~+ cell population accounts for 13.08±1.39% of total cells in all carcinomas. No correlation between the number of CD133~+ cells and tumor stage or grade or patient age or sex was observed. The purity of CD133~+ cells was about 85±2.4% and the viability of cells was not affected by the MACS. Comparing the expression of CD133~+ cells with CD133~- cells, there were eight genes increased and twenty-three decreased. The main difference of expression of microrray was on genes BMP2K,CD44,IGF1,TDGF1 and ABCG2.Conclusions The percentage of CD133~+ progenitor cells of renal carcinomas was about 13.08±1.39%, the CD133~+ progenitor cells probably were the initiating cells of renal cell carcinomal. The method of MACS is feasible for the isolation of renal cancer stem cells. The product of genes BMP2K,CD44,IGF1,TDGF1 and ABCG2 located on cell membrane may be a useful marker to isolate and purify kidney cancer stem cell.