Research Of Endothelization On Titanium Oxide Film Surface Modified By Laminin Immobilization

In this work, titanium oxide (Ti-O) films were synthesized using Unbalance Magnetron Sputtering (UBMS). After the Ti-O thin films were pretreated using NaOH solution for activity of hydroxyl, aminopropyltriethoxysilane (APTE) was interacted with the hydroxyl on the Ti-O film surface from activation. Then, laminin (Ln) were covalently immobilized to the film surface by the amidogen of APTE. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier Transform Infrared spectroscopy (FTIR), Atomic Force Microscopy (AFM) and contact-angle measurement were used to investigate the characteristics of the surface of Ti-O films and modified Ti-O films. Human umbilical vein endothelial cells (HUVEC) were in vitro seeded to the modified and unmodified Ti-O films surface for evaluating the cell compatibility and endothelialization trend, and the experiments of laminin directly immobilizing on the surface of Ti-O film were also performed. In addition, the behavior of blood platelet adhesion was studied by SEM after incubation of the samples in platelet rich plasma (PRP).The results of FTIR indicated that the functional group of hydroxyl was presented onto Ti-O film surface after being pretreated by NaOH. The peak of O element corresponding to hydroxyl group increased in results of XPS, the contact angle of Ti-O film surface was lower.The results of FTIR and XPS indicated that APTE could be chemically reacted with the hydroxyl of Ti-O film surface. The results of SEM and AFM suggested that the surface morphology has not changed after APTE coated and the contact angle of the film surface increased. The results of FTIR indicated that amide I, amide II and -NH2 adsorption peaks was presented after laminin immobilized onto Ti-O films. The peak of sulfur (S) element was presented in results of XPS. Those results suggested that laminin could be immobilized to Ti-O film surface by APTE. And the contact angle of Ti-O film surface was lower, the hydrophilicity of film was increased.Cell culture investigation suggested that laminin could enhance HUVEC adhesion and proliferation, and improve the HUVE cell behavior. Directly immobilization of laminin on the surface of Ti-O film also showed good cell compatibility. And the results of HUVECs cultured experiment suggested that Ti-O film which modified by APTE and immobilized with Ln is better than that of Ti-O film immobilized directly with Ln.The results of blood platelet adhesion experiment indicated that after covalently immobilizing of Ln on APTE coated Ti-O film surface, behavior of blood platelet adhesion became better.All these results indicated that laminin could be immobilized onto Ti-O film surface by APTE. Laminin can promote the adhesion and growth of endothelial cells, and behavior of blood platelet adhesion became better. So, it is a good surface endothelialization way to immobilized laminin onto Ti-O film surface.