Titanium Oxide Surface Modified By Plasma Immersion Ion Implantation Using Ammonia And Laminin Immobilization

In this paper, Ti-O films were synthesized by Unbalance Magnetron Sputtering on silicon substrates,and then treated by plasma immersion ion implantation (Pâ…¢) using ammonia gases in order to introduce amino group to Ti-O film's surface. Based on preliminary work, the implantation technology was optimized and the pulse width, frequency, voltage and implantation time were changed in order to study how the changing of implantation parameters influenced theimplanted dose of anmino. The structure and the chemical composition of the surface before and after implantation and the relative content of anmino were analysed by Scanning Electron microscope (SEM), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR), fluorescence labeling method and X-ray photoelectron spectroscopy (XPS). Then compared the anmino treated by the plasma immersion ion implantation with the anmino treated by silanization. On this basis, laminin (Ln) was immobilized to the surface of the Ti-O film which has the relatively more amino content, In the end, human umbilical vein endothelial cells (HUVEC) primary culture experiments were adopted in vitro to evaluate the compatibility of the endothelial cells on the surface of the untreated Ti-O films,Pâ…¢-treated Ti-O films and the Ti-O films immobilized by biomolecules. The morphology of endothelial cells was observed by optical microscopy.The contact angle of Pâ…¢-treated Ti-O films surface was higher than that of the untreated Ti-0 films. The fluorescence labeling method and XPS results show that the functional group of amido has been introduced to the surface of Ti-O film by plasma immersion ion implantation. With the adjustment of implantation parameters, the content of anmino implanted into the sample surfice is different. The results of SEM and AFM suggest that the roughness of the Pâ…¢-treated Ti-O films surface has been increased. The result of immunofluorescence indicates that laminin has been immobilized to the Pâ…¢-treated Ti-O film surface successfully.Endothelial cell culture experiment suggests that, Ti-O film itself has a certain endothelial cellular compatibility and the amino groups are helpful for the adhesion and proliferation of HUVEC. The Laminin can further enhance HUVEC's growth.All these results indicated that the ion implantation can introduce functional group onto the surface of Ti-O film. What's more, with the change of pulse width, frequency, voltage and implantation time, the content of anmino was different. Also the ion implantation can change the properties of the Ti-O film like wettability and roughness. It can promote the adhesion and growth of endothelial cells to immobilize Laminin onto the surface of Ti-O film by amino group.