| Objective: To explore the effect of neuron stem cells (NSCs) transplantation on promoting therecovery of the hindlimb motofunction and the expression of neurotrophins (NTFs) followingspinal cord transection in adult rats.Methods: 30 adult femal SD rats were randomly divided into 5 groups: sham-operated group,spinal cord transected (spinal cord was completely transected between T9-T10 segments) groups,which survived 7 days and 14 days, and neuron stem cell transplanted (placed a small piece ofgelfoam, which was preinjected with cell suspension containing neural stem cells, in thetransected site of the spinal cord) groups, which survived 7 days and 14 days. the recovery ofmotofunction in each groups were evaluated by Basso Beattie Bresnahan (BBB) scoring at 7and 14day respectively, the Spinal cord segment of one rat in each group was used to study thesurvival and migration of the transplanted NSCs. The other five animals' spinal cord were usedfor Western blotting analysis the changes in the expression of NGF, BDNF, NT-3 and NT-4protein in the injuried spinal cord. Densitometric analysis was performed using Bio-GelImagining system equipped with Genius synaptic gene tool software. Datas were analyzed byone-way ANOVA and LSD-q testResults: 1. The BBB scoring in the operated group and NSCs transplanted group were 0.8 and2.2 respectively at 7 days. And at 14days, the operated group and NSCs transplanted groupscore reached 3.8 and 6.6. the BBB scoring in NSCs transplanted groups were higher than theoperated groups, their motofunction were improved evidently after NSCs transplantation. 2. thetransplanted NSCs could survive and migrate in the spinal cord. 3. Western Blot analysisshowed that: the expression of NGF, BDNF, NT-4 didn't change after transection at 7days and14days(P=0.549, 0.958, 0.183, 0.868, 0.374, 0.685). but the expression of NT-3 decreased(P=0.000,0.001). After NSCs transplantation for 7 days, the expression of NGF increasedrapidly (P=0.001),although the expression of BDNF decreased ,the significance is 0.153, and the expression of NT-3 and NT-4 didn't change (P=0.521, 0.621). After transplantation for14 days, the expression of NGF was still higher than the operated group,but the significance is0.078; the expression of BDNF decreased (P=0.032); there were no changes in the expressionof NT-3 andNT-4 (P=0.108, 0.621).Conclusion: 1. NSCs transplantation can promote the recovery of motofunction in adult ratsafter spinal cord transection.2. The transplanted NSCs can survive and migrate in the injuried spinal cord.3. The changes in the expression of NGF, BNDF, NT-3 and NT-4 proteins weredifference after NSCs transplantation. The expression of NGF increased andBDNF decreased after NSC transplantation and there were no changes in theexpression of NT-3 and NT-4 after NSCs transplantation. All these studiesindicated that NSCs transplantation may improve the hindlimb motofunctionof the transected rats related with the changes in the expression of NGF andBDNF.
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