Culture And Clinical Meaning Of Human Fetal Olfactory Ensheathing Cells

【Objective】To develop the better methods of drawing the materials from OB and purifying OECS.【Methods】We drawing the materials from OB by crushing directly. We had adjusted inoculated density of OECs to uniform 10~5/ml, We made a contrast of cells purity of each 10 samples disposed by three purification methods respectively; The primary cells which possessed the same density and the same inoculating quantity was fetched 10 portions respectively to implant in three kinds of Petri dish. We checked out cellular anchoring velocity and final purity.【Results】We considered the immunoadsorption as the best method of purifying OECs, whose cells purity achieved 84.66%. But differential anchoring (71.59%) and chemical technique (66.45%) were not desirable because of losing a great deal of dissociated cells; Coating the petri dish was profitable to purify the cells, p75 coating petri dish could not only culture the highest cell purity (88.69%), but also help OECs to anchor rapidly.【Conclusion】A large number of pured OECs will provide convenience for further basal study and clinical application in future.