The Research Of Glucose Transporter4 MRNA In The Myocardial Cell In The State Of Insulin Resistance

PrefaceInsulin resistance(IR) is the mechanism that leads to the disorder of human metabolism. Evidences demonstrate that hypertension, atherosclerosis, and cardiac vascular heart is on the pathogenic basis of insulin resistance. The diabetogenic myocardosis results from the metabolic disorder of myocardial cell. The glucose transporter4(Glut4) is the important transporter that transport glucose from outside of cell to inside. Glut4 is distributed in strip muscle cell and myocardial cell. Recent studies show that the expression of Glut4 in strip muscle cell decreases in the state of IR, but there are few studies about the expression of Glut4 in myocardial cell in the state of IR.Materials and MethodsWe purchase 40 Wistar male rats from experimental animal center of China Medical University. The rats are divided in three groups according to random principle. We feed the rats in Group One on the normal diet,which contains 21% protein, 19% fat and 60% carbohydrate. We feed the rats in Group Two on the more fat diet, which contains 21% protein, 60% fat and 19% carbohydrate. We feed the rats on the more fat and more sugar diet, which contains 21% protein, 60% fat, 19% carbohydrate and 40 grammers extra sugar. We have cultivated three groups of rats for eight weeks in order to build the rat models that are in the insulin resistance state.We examine insulin resistance in the experimental rats by clamp technique. We get the serum samples of these rats afer clamp technique examination. We examine the serum blood sugar . Afer killing the rats we get the myocardia samples, which are stored under 4% PFA. All samples are preserved in low temperature. Myocardia samples are routine paraffin imbeddinged after dehydration, transparent, immerse wax as immunochemistrical samples. The expression of Glut4 in the myocardial cells is examined by immunochemical method. Continuous distribution measurement data is demonstrated with mean±S.D. We proceed ANOV on them by. All statistic analysis are completed by SPSS package.Result1. Body Weight: the Wistar rats in Group Two and Group Three are heavier than Group One(P<0.05), and the Wistar rats in Group Three is heavier than Group Two(P<0.05);2. Blood Sugar(BS): there is no statistical difference in the BS of the rats among the three groups(P>0.05);3. Glucose Intake Rate(GIR): the GIR of the rats in Group One is higher than Group Two and Group Three(P<0.05) and the GIR of the rats in Group Two is higher than Group Three(P<0.05);4. The expression of Glut4 mRNA in myocardial cell: the expression of Glut4 mRNA in myocardial cell of the rats in Group One is higher than Group Two and Group Three(P<0.05) and the the expression of Glut4 mRNA in myocardial cell of the rats in Group Two is higher than Group Three(P<0.05);5. Relation Analysis: GIR has negative correlation with body weight and the coefficient of correlation is -0.849, (P<0.05); the expression of Glut4 mRNA in myocardial cell has positive correlation with GIR and the coefficient of correlation is 0.819, (P<0.05).Conclusion1. The high level of fat in the diet of the high level of sugar and fat in the diet results in insulin resistance;2. The expression of Glut4 mRNA in myocardial cell decreases in the state of IR.