Expression Of Tenascin And CD34 In Human Esophageal Squamous Carcinoma

ObjectiveEsophageal carcinoma is normal in human malignant tumor, and is characterized by it's invasions, infiltrations, disseminations through lymph node and blood. The tumor infiltrates easily because the absence of serosa, so the prognosis of patients that have esophageal carcinoma is poor. The recurrence and metastasis even happen soon after operation. It's not concord to the available information. Screening the patients those recur and metastasis more easily has an important clinical significance. For the past few years, it's found that the extracellular matrix (ECM) has a close relationship to the growth and metastasis of tumors. The tenascin is a hexameric extracellular matrix glycoprotein. It expresses transiently in many developing organs and often re-expressed in tumors. But it hasn't been reported in esophageal carcinoma. Angiogenesis is important in the progression of tumors, and the microvessel density (MVD) may presents the activity of neovascularization.CD34 is a sensitive marker for blood vessels. Detecting CD34 in carcinoma tissue may reflex MVD. In my study, TN and CD34 are detected to evaluate their clinical pathology, and to help the diagnosis and treatment of carcinoma.Material and method1. The source of cases91 cases of the esophageal carcinoma tissues and 47 cases of normal esophageal tissues are from the department of chest surgery of ShengJing hospital of China Medical University from Oct,2004 to Feb,2006.2. Method Immunostaining of TN and CD34 in 91 cases of carcinoma tissue respectively, and 47 cases of normal tissue as TN's control group, 10 cases of normal tissue as CD34's control group.3. Standard of consequence(1) TN expresses in the matrix of carcinoma tissue in dark brown, and measuring their OD values by microgramme analytical system.(2) CD34 stains endothelial cell into dark brown, which refers to the Weidner's methods.4. Statistics analysisSpss 12.0 is used to analysis the data. T-test is used to analysis the difference between case and contral group in TN and CD34 respectively. The group comparison of clinical pathological characteristic of TN and CD34 in esophageal carcinoma is analyzed by the analysis of variance. Spearman is used to analysis the relationship between TN and CD34. A p value of less than 0.05 was considered significant.Result1. Expression of TN and CD34 in esophageal carcinoma(1) The expression of tenascin in esophageal cancer was much stronger than that in the normal tissue (t=12.331,P＜0.01).(2) The expression of CD34 in the esophageal carcinoma tissues was stronger than that in the non-esophageal carcinoma tissues (t=6.023, P＜0.01)2. The relationship between TN or CD34 and clinical pathology characteristic(1) Over expression of TN was related to the length (F=12.373, P＜0.01), the invasion (F=11.039, P＜0.01), the lymphatic metastasis (F=6.882, P =0.01) and the pathologic grade (F=5.060, P =0.003) of cancer.(2) Over expression of MVD was related to the length (F=9.033, P＜0.01), the lymphatic metastasis (F=12.429, P＜0.01) and the pathologic grade (F =5.717, P＜0.01) of cancer. 3. The relationship between TN and CD34The positive expressions of TN and MVD were associated (P =0.001).Conclusion1. The expression of TN and CD34 in case and control group have a significant difference.2. TN's overexpressions may be a biological marker in esophageal squamous cell carcinoma. The levels of TN were useful molecular markers for evaluating malignancy degree and lymph node metastasis of esophageal carcinoma.3. CD34's overexpressions may be a biological marker in esophageal squamous cell carcinoma. The levels of CD34 were useful molecular markers for evaluating malignancy degree and lymph node metastasis of esophageal carcinoma.4 TN is associated with the expression of CD34.