Study On Expression Of Osteopontin And Relation Between Osteopontin And Tumor Angiogenisis In Esophageal Squamous Cell Carcinoma

Objectives: 1. To observe the expression of osteopontin(OPN) in tumor tissue, tumor adjacent tissue and metastasized lymph nodes in esophageal squamous cell carcinoma, to explore the relation between OPN and clinicopathological characteristics, further to explore the effect of OPN in lymph nodes metastasis. 2. To observe the expression of CD34 and count microvessel density in esophageal squamous cell carcinoma, to explore the effect of OPN in tumor angiogenesis. Methods: 1. S-P immunohistochemical staining was used to investigate the expression of OPN by anti-osteopontin polyclonal antibody, and the specimens were 44 cases of tumor tissue, tumor adjacent tissue and 20 cases of metastasized lymph nodes of esophageal squamous cell carcinoma. 2. S-P immunohistochemical staining was used to investigate the expression of CD34 in the specimens of 44 cases of esophageal squamous cell carcinoma by anti-CD34 polyclonal antibody, and microvessel density was counted in the high power field (×400) of microscop. Results: 1. The total positive rate of OPN expression in 44 cases of esophageal squamous cell carcinoma is 86.3%. There was no positive expression of OPN in tumor adjacent tissue of 44 cases, and in 20 cases of metastasized lymph nodes the positive rate of OPN was 100%. OPN was mainly distributed in cytoplasm of tumor cells. The distribution of OPN was rich in carcinoma cell nests diffusely. The result showed that there was a significant difference for OPN expression between clinical stage Ⅰ-Ⅱ and Ⅲ(P<0.05). And so did the group N0 and group N1 in lymph node metastasis status (P<0.05). The statistic analysis didn't show any significant relationship in location of tumor, diameter of tumor, tumor infiltration and pathological grading for expression of OPN. There was also a statistical significant difference for OPN expression among tumor tissue, tumor adjacent tissue and metastasized lymph nodes (P<0.05). 2. The microvessel density counting of 44 cases of esophageal squamous cell carcinoma is 24.07±5.82. The microvessel of carcinoma nests was less than the area around it. The result showed that there was significant relationship in clinical stage, tumor infiltration and lymph nodes metastasis for MVD. But the statistic analysis didn't indicate any significant relationship in location of tumor, diameter of tumor and pathological grading for MVD. It was found that the MVD of OPN expression in positive was higher than that in negative. Rank correlation analysis showed that OPN and MVD were positive correlation. Conclusion: 1. The distribution of OPN was rich in carcinoma cell nests diffusely, and OPN was mainly distributed in cytoplasm of tumor cells. It indicated that OPN was mainly produced by tumor cells. The expression of OPN was closely correlative to clinical stage and lymph nodes metastasis. It indicated that OPN was closely correlative to tumor progression. 2. There was active angiogenesis in esophageal squamous cell carcinoma and angiogenesis had a close correlation to clinical stage, tumor infiltration and lymph nodes metastasis. The MVD of OPN expression in positive was higher than that in negative. Rank correlation analysis showed that OPN and MVD were positive correlation. It indicated that OPN had close relationship with tumor angiogenesis in esophageal squamous cellcarcinoma.