Study On Detection Methods Of Gold Nanoparticles, Terbutaline Sulfate And Uric Acid By Capillary Electrophoresis Coupled With Chemiluminescence Detection

Capillary electrophoresis (CE) is a powerful analytical technology with high separation efficiency, less time-consuming, simple instruments operating, low-cost samples and available automation. CE has many virtues in the separation of many substances including proteins, DNA, sugar, small ions, mast cells even the contents of sing cell, and has successfully coupled with a variety of detectors, such as ultraviolet (UV), laser-induced fluorescence (LIF), electrochemical (EC), mass spectrometry (MS), electrochemiluminescence (ECL), chemiluminescence (CL). CL is one of the most sensitive detection methods in CE, without light sources requiring, less background disturbing, simple optical systems, available operation and wide linear range. CL is broadly applied in the domain of environmental science, clinic medicine, pharmaceutic analysis and industrial analysis in micro-quantum and trace quantum range. When coupled with CE, it can meet the demand of high separation efficiency and high sensitivity for the complex samples. It is broadly applied in the fields of metallic ions, amino acids, proteins and polypeptide, adenosine triphosphate, alkaloids, druggery and so on.Nanoparticles with special physical and chemical properties have broad applicable prospects in the fields of optics, electrics, magnetics, catalytic reaction, biomedicine and material sciences. Accurate and rapid detection and characterization of nanomaterials is of vital significance for promoting the development of nanotechnology.Pharmaceutic analysis mainly has spectrophotometry and liquid chromatography. Complex biological samples usually needs to be treated before experiments. Compared with spectrophotometry and liquid chromatography, the samples and reagents in CE is cost least. So, establishing a rapid, accurate and sensitive detection method of drugs is of important significance in its pharmacology, pathology and clinical research. Through the direct detection of some unknown substances in biological fluids and via evaluating the contents, we can draw a conclusion on the disease diagnoses and treatments. Therefore, establishing a rapid, accurate and sensitive detection method is of great practical significance in its clinical research. In this work, the self-assembly CE-CL instruments are utilized in the course of total experiments. The studies on detection methods of gold nanoparticles, terbutaline sulfate and uric acid by CE coupled with CL detection have been exhibited. The main contents in the thesis consist of the following three parts:Part one: Based on CE-CL, a new method for determination of gold nanoparticles in the CL reaction of luminol with hydrogen peroxide in sodium hydroxide medium was set up. With the optimal condition, several grain-sized gold nanoparticles were carried through. The results demonstrates that these nanoparticles all catalyze the CL reactions, the catalysis phenomenon of 38 nm grain-sized gold nanoparticles is most sensitivity in all kinds of gold nanoparticles, and their CL intensities have good linear relationships with their concentrations in some concentration range, respectively.Part two: Based on CE-CL, with the enhancement of terbutaline sulfate in the CL reaction of luminol with potassium ferricyanide in sodium hydroxide medium, with the optimal condition, a new method was established for the determination of the terbutaline sulfate. The linear range was 7.2×10-8~3.6×10-6 mol/L with the detection limit (S/N = 3) of 3.0×10-8 mol/L. Relative standard deviation (RSD) was 4.6 % for 3.6×10-6 mol/L terbutaline sulfate (n = 11). This method was applied to the determination of terbutaline sulfate in commercial tablets. Meanwhile, the possibility of determination was explored in the human urine, the experiments showed that the detection of terbutaline sulfate was not influenced in the environment of human urine. The recoveries were 100 %~105 %.Part three: Based on CE-CL, with the enhancement of uric acid in the CL reaction of luminol with potassium ferricyanide in sodium hydroxide medium, with the optimal condition, a new method was founded for the determination of uric acid. The linear range was 3.0×10-5~6.0×10-7 mol/L with the detection limit (S/N = 3) of 3.5×10-7 mol/L. RSD was 3.5 % for 6.0×10-6 mol/L uric acid (n = 11). The method was applied to the determination of uric acid in human serum and urine. The experiments showed that the detection of uric acid was not influenced in the environment of biological fluids with satisfactory results.