The Effect Of Antisense Oligonucleotide Targeting C-myc On The QBC-939 Cholangiocarcinoma Cell Development And Sensitivity To Chemotherapy

Objective:By transfecting c-myc antisense oligonucleotide(ASODN) into QBC-939 cells, we explore the effect of c-myc ASODN on cell cycle,apoptosis and radiotherapy sensitivity.Methods: 1.QBC-939 cells were cultured routinely; 2.ASODN targeting c-myc was designed and constructed; 3.Cultured cells were divided into 8 groups:control group, lipofectin group ,5umol/L sense oligonucleotide(SODN) group, 10umol/L SODN group, 15umol/L SODN group,5umol/L ASODN group, 10umol/L ASODN group and 15umol/L ASODN group. After transfection for 72h,cultured cells were harvested to carry on the next tests; 4.C-myc protein expression was detected by immunohistochemistry; The changes of cell cycle and apoptosis rate were examined by flow cytomet, rate of inhibition (IR) by chemotherapeutic drugs was determined by the colorimetric MTT cell viability/proliferation assay.Results:l.Expression of c-myc protein in ASODN groups was significantly decreased compared with that in the control, lipofectin and SODN group; 2.The percentage of G1 QBC-939 cell was significantly increased compared with other group. The percentage of S QBC-939 cell was significantly decreased compared with other group; 3.The apoptosis rate of ASODN groups was significantly higher than that of other groups; 4.The IR of chemotherapeutic drugs in ASODN groups was significantly higher than that of other groups.Conclusions:Expression of c-myc may decrease in QBC-939 cells after ASODN transfection. ASODN targeting c-myc can inhibit cells from G1 phase into S phase,induce cells apoptosis,and sensitize cholangiocarcinoma cells to chemotherapy.