| Objective: With the development of social economy and the improvement of life level in recent years,the diet has been changed enormously. The morbidity of hyperlipoidemia has increased largely because of the high sucrose and high fat diet and the less body activity or obesitas and so on .Hyperlipidemia was defined as serum total cholesterol (TC) and triglyceride(TG) increased.It includes hypertriglyceridemia, hypercholesteremia and high-density lipoprotein cholesterol (HDL-C) decreased. Hyperlipidemia is one of the principal risk factors in the development of atherosclerosis. Coronary heart dlisease (CHD) is the leading cause of mortality and morbidity in many countries .For the past few years, Traditional Chinese medicine has great effects on adjusting blood fat metabolism and preventing atherosclerosis and coronary heart disease. Rhodiola sacra extract from the integripetal rhodiola herb on highland. Many people carried on clinical tests and animal experiment give full proof of the fact that that Rhodiola sacra can reduce blood fats and prevent coronary heart disease. It is used widespread in preventing and treating coronary artery disease, angina pectoris, myocardial ischemia and other disease of blood stagnation in clinic. The therapy has been proved effective and adverse reactions haven't been found. But the mechanism of Rhodiola sacra extract in treating hyperlipidemia need further investigation .In 1990 year, Issemann find a member of the steroid hormone receptor superfamily by peroxisome proliferators. It called Peroxisome proliferators-actived receptor(PPAR). Some fatty acids and Peroxisome proliferators can activate PPAR. PPAR have diverse biological functions and play an important role in lipid metabolism,inflammatory process ,immunologic reaction and so on, which is the focus of much of the researches at present. PPAR-s have three subtypes: PPAR-α,PPAR-γand PPAR-β. PPAR-αis expressed most in liver, then kidney, heart and skeletal musele. PPAR-γis expressed most in adipose cell. Peroxisome proliferators-activated receptors(PPARS) are members of the nuclear receptor superfamily. It may induce adipocyte differentiation and regulate the metabolism of lipids. It may be the physiosensor of lipid and play great effects on the dynamic equilibrium of lipids metabolism .In this research, we fed male Wistar rats with high fat diet to make hyperlipoidemia rat model. To objective the effect of integripetal rhodiola herb extract on the expression of PPAR-α, PPAR-γin Liver, in order to interpret the mechanism of the integripetal rhodiola herb extract on lipid metabolism.Method: 30 adult, healthy, clean, male Wistar rats, 16~20 weeks old, 250~300g weight, were purchased from experimental animal center in Hebei medical university and acclimatized for 2 weeks. Animals were randomly divided into normal control(NC)group, high-fat diet (HF) group. NC group(n=10) were fed with basic diet. The calorie constitution is that of calories from 65.5% for the carbohydrate, 10.3% for the fat, 24.2% for the protein; high-fat diet (HF) group were fed high-fat diet. The calorie constitution is that of 20.1% for the carbohydrate, 59.8% for the fat, 20.1% for the protein. The oil, butter all came from experimental animal center in Hebei medical university. At the end of 4th weeks, HF group were random divided into two equal groups :rats without rhodiola sacra extract treatment, that is hyperlipidemia group (HL), and rats with rhodiola sacra extract, that is hyperlipidemia and integripetal rhodiola herb extract group(HLR). integripetal rhodiola herb extract 1 g·kg-1·d-1 were given orally to HLR. Equal amount normal saline was given orally to HLR group. Animals were fed for 8 weeks. Body weight (BW), systolic blood pressure (SBP), serum total cholesterol (TC), serum triglyceride (TG), serum free fatty acids (FFA) were observed every 4 weeks. Animals were put to death at the of 8 weeks. And the level of PPAR-α, PPAR-γmRNA in Liver were measured by reverse transcription polymerase chain reaction technique(RT-PCR). The correlation among the FFA, TC, TG, and the level of PPAR-α, PPAR-γin Liver was studied.Result: 1.Body weight in HF group increased, but there were not difference than that of in NC group(p>0.05) after 4 weeks. Body weight in HLR group didn't increase obviously, showed no difference between NC groups(p>0.05) and no difference than of in NP group(p>0.05)after 8 weeks.2.Blood pressure in HF group increased obviously, there were significantly difference compared with NC group(p<0.05)after 4 weeks. Blood pressure in HL group increased obviously, there were significantly difference compared with NC group(p<0.01)after 8 weeks. Blood pressure in HLR group decreased obviously, showed significantly difference between NP groups(p>0.05) but no than of in NC group(p<0.01)after 8 weeks.3.TC, TG in HF group increased obviously, there were significantly difference than that of in NC group(p<0.05)after 4 weeks. TC and TG in HL group showed significantly difference than of in NC group(p<0.01)after 8 weeks. TC and TG in HLR group decreased obviously, showed no difference between NC groups(p>0.05) but significantly difference than of in NP group(p<0.01)after 8 weeks.4.FFA increased significantly in NF groups compared with that in NC group after feeding for 4 week (p<0.01). FFA in HLR and NC group obviously lower than that in HL group (p<0.01) at 8 week, but there were no difference among HLR and NC group(p>0.05).5. The level of PPAR-αmRNA in HL group rat liver is obviously lower than in NC group after 8 weeks. The level of PPAR-γmRNA in HL group rat liver showed no difference between NC groups(p>0.05)after 8 weeks. The level of PPAR-αmRNA and PPAR-γmRNA was markedly increased in all experimental groups compared with the NC group (p<0.01) after 8weeks.6. The level of PPAR-αmRNA in Liver was negatively correlated with FFA, TC, TG and BP. The level of PPAR-γmRNA in Liver showed no obvious correlation with FFA, TC,TG and BP .Conclution: 1.Respectively fed with high fat diet displayed hypertriglyceridemia, hypercholesteremia and high blood pressure; so Wistar rats with hyperlipoidemia were induced successfully.2.FFA, TC, TG and BP in HL group increased obviously, there were significantly difference compared with NC group(p<0.01)after 8 weeks. FFA, TC, TG and BP in HLR group decreased obviously, showed significantly difference between NP groups(p>0.05)but no than of in NC group(p<0.01)after 8 weeks.3.The level of PPAR-αmRNA in HL group rat liver is obviously lower than that of in NC group after 8 weeks. The level of PPAR-γmRNA in HL group rat liver showed no difference between NC groups(p>0.05)after 8 weeks. The level of PPAR-αmRNA and PPAR-γmRNA was markedly increased in HLR groups compared with the NC group (p<0.01) after 8 weeks.4.The level of PPAR-αmRNA in Liver was negatively correlated with FFA,TC,TG and BP. The level of PPAR-γmRNA in Liver showed no obvious correlation with FFA, TC, TG and BP. These results suggested that the change of the level of PPAR-αmRNA was related closely with hyperlipoidemia.
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