Serum C-reactive Protein Levels In Subjects With Varying Tolerances And The Relativity Study Of SE-selectin

Objective:Vasculopathy is the main chronic complications of diabetesmellitus.Macroangiopathy is the main cause of death of diabetic patients.The pathogenesis of diabetes mellitus and its macroangiopathy isn'tcompletely clarity yet. C-reactive protein (CRP) is a accepted systeminflammation marker, the level of CRP is remarkablely high in patient ofdiabetes especially with vascular complication. This study is aimed atinvestigating the difference of serum C-reactive protein(CRP)concentrations in subjects with various glucose tolerance and observationthe correlation with sE-selectin to supply experimental base for theeffect of CRP on the endothelium cell function and explain that whethersystem inflammation participate the onset of vascular complication indiabetic patients.Methods:1. Physical examination was given on every subject(112 case) with historyof disease recorded, blood sample taken. (NGT: 34 case; IGT: 36 case;T2DM 42 case).2. Serum CRP level was detected with immunal turbidity method in NGT,IGT,T2DM;sE-selectin level was detected with ELISA method; Blood lipid,liver and kidney function was detested with enzyme method. Blood sugarwas detected with the glucooxidase method; Serum insulin wasdetermined with the radio immunity method.3. Evaluate the insulin resistance level with the homeostasis model(HOMR-IR). HOMA-IR= (FINS[μU/mL]×FPG [mmol/L])/22.5.4. The first part: collect date of the above three crowd. Analysis therelationship between hs-CRP and impaired glucose tolerance; The second part: delamination analysis was done in T2DM: First, Good control groupand bad control group come out by the level of HbA₁c;second, the modestinsulin resistance and severe insulin resistance are distinguished byHOMA-IR;Third, obesity and non-obesity are distinguished by BMI.Approaching the relativity between CRP and sE-selectin.5. All data was analyzed with SPSS 13.0 software. When comparing threefactors, analysis of variance (ANOVA) was used;When comparing twofactors, analysis of two samplet test was used;When analysis ofcorrelation between two factors was performed using Pearsoncorrelation;When analysis of correlation all parameters, multipleregression was used.Results:1. The serum CRP concentration was 3.49±0.59mg/l in NGT subject, 3.93±0.82mg/l in IGT subject, 4.47±1.07 in T2DM patients; It'sconcentration is gradually increased and have statisticalsignificance (P＜0.01).2. We do delamination analysia in T2DM. The serum CRP and sE-selectinhave same change tendency. The concentrations are high in badcontrolled patients (hs-CRP: 5.13±0.99; sE-selectin: 8.11±0.79)than good controlled patients (hs-CRP: 4.08±0.92: sE-selectin:7.28±0.71) ;The concentrations are high in serious insulinresistance(IR) patients(hs-CRP: 5.07±1.00: sE-selectin: 8.07±0.75)than gently insulin resistance(IR) patients (hs-CRP: 4.24±1.02;sE-selectin: 7.40±0.80) ;The concentrations are high in obesitypatients (hs-CRP: 4.82±0.95; sE-selectin: 7.81±0.78) thannon-obesity patients (hs-CRP: 4.22±1.10; sE-selectin: 7.43±0.85).3. The serum CRP concentration was positively correlated with HbA₁c(r=0.497, p＜0.05),HOMA-IR (r=0.505, p＜0.01) in T2DM; The serumE-selectin concentration was positively correlated with HbA₁c(r=0.546, p＜0.05),HOMA-IR (r=0.505, p＜0.01). 4.The serum CRP concentration was positively correlated withsE-selectin concentration (r=0.905, p＜0.01) in T2DM.Conclusion:1. The serum CRP concentration is gradually increased with the worseningof glucose.2. The change of serum CRP concentration and the change of sE-selectinconcentration have relativity.