Study On Exploiting A Device For Bilirubin Ambulatory Detection And The Capacity Of Dynamic Adsorption Bile By Smectite In Vitro

Objective:The aims of this paper are to exploit a device for 24 hours bilirubinambulatory spectrophotometric detection, comparing with Bilitec 2000 invitro, and to study the adsorbing capacity of smectite to total bilirubinand total bile acid in vitro.Methods:1. A device for 24 hours bilirubin ambulatory detection was designedon spectrophotometry.2. Both Bilitec 2000 and our self-designed device were used to detectthe absorbency of bilirubin solutions of varieties concentrationsranging from 500μmol/L to 3.90625μmol/L.3. The bile was taken from a patient, who had undergone PTCD, wasdiluted to different concentrations which were then titrated to the pHvalue at 1,3,5 and 7 respectively. All of the bile samples were interfusedwith 150mg/ml smectite, and centrifuged. Then, the supernatant fluid wastitrated until its pH value reached 7 and its concentrations of totalbilirubin and total bile acid were mensurated.Results:1. A device for 48 hours bilirubin ambulatory spectrophotometricdetection had been exploited by us.2. It was found there were positive correlations between Bilitec 2000and our self-designed device (R=0.982, P=0.000) in respect ofmensurating the concentrations of bilirubin. The relation of theabsorbency detected by our device and the concentration of bilirubin was(?):-0.1226+0.0982lnX (F=215.59, P=0.000, R~2=0.840). 3. It was found that the total bilirubin in bile was adsorbedcompletely bysmectite, and there were negative correlations between thecapacity of adsorption total bile acid and the concentrations of totalbile acid and negative correlations between the capacity of adsorptiontotal bile acid: (?)=0.899-0.002*C-0.063*pH (R=0.953, P=0.000).Conclusions:The device for 24 hours bilirubin ambulatory spectrophotometricdetection, which was exploited by us, had the same capability as Bilitec2000, so it should be used in clinic.The result of negative correlations between the capacity ofadsorption total bile acid and the pH values given a clue that smectitemight adsorb bile acid in stomach and then release part of it in smallintestine so that smectite could protect the mucous membrane of digestivetract and have small effect of enterohepatic circulation of bile acid.When smectite were used to cure patients with duodenal gastroesophagealreflux disease, the result of negative correlations between the capacityof adsorption total bile acid and the concentration of total bile acidgiven a clue that dosage dependence might be in existence.