The Relationship Between The Infection Of Hepatitis B Virus Mediated By Placental Complement Receptor And The Intrauterine Transmission Of Hepatitis B Virus

Objective: To study the mechanism of intrauterine HBV infection.Methods: Partâ… 1 73 placentas of HBsAg positive pregnant women were chosen.The HBsAg were detected by PV-9000 immunohistochemical staining in these placentas.2 The distribution of C3c in HBsAg positive placentas were detected by immunofluorescence assays and the confocal laser scanning technique.3 The distribution of HBsAg and C3c in C3c positive placentas were detected by double-label immunofluorescence assays and the confocal laser scanning technique.Partâ…¡1 10 cases of placentas from normal(maternal serum HBsAg negative) pregnant women were collected at the term, another 10 cases of term placentas, were selected randomly from 47 HBsAg positive placentas. The distribution of placental complement recetpor in two groups were detected by immunofluorescence assay and the confocal laser scanning technique.2 The distribution of HBsAg and complement receptor in HBV positive placentas were detected by double-label immunofluorescence assays and the confocal laser scanning technique.3 The clinical datas of pregnant women and their newborns in part one were collected. The peripheral blood hepatitis B virus markers(HBV-M) of 73 newborns were detected within 24 hours after born, at 1-month and 6-month. Based on the results of part one, all the cases were divided into either HBsAg/CR positive group or HBsAg/CR negative group, comparing with the difference of intrauterine HBV infection, and comparing with whether using hepatitis B immunoglobulin antepartum is different between the two groups. Results: Partâ… 1 There were 47 HBsAg positive placentas in total 73 cases(the placental HBsAg positive rate is 64.3ï¼…). The positive signal was existed in trophoblastic cells, villous mesenchymal cells and vascular endothelial cells2 There were 33 placentas, in which complement 3c were positive, in total 47 HBsAg positive cases.3 HBsAg and C3c could exist in the same sites of placental cells together, and they were mainly distributed in the trophoblastic cell membranePartâ…¡1 Complement receptor exists in the placentas of both normal and HBsAg positive placentas, they exist in trophoblastic cells, and villous mesenchymal cells.2 HBsAg and complement receptor could be detected in the same sites of placental cells together, and they exist in the trophoblastic cell membrane mostly.3 There is no statistical significance in whether using hepatitis B immunoglobulin antepartum or not between HBsAg/CR positive group and HBsAg/CR negative group, and meanwhile there is no statistical significance in intrauterine infection between the two groups.Conelusion:1 Complement receptor exists in human placenta,and the expression of complement receptor is not related to the placental HBV infection.2 HBsAg-anti-HBs-complement C3c compounds exist in the HBsAg positive placentas.3 The route of infection mediated by complement receptor on placental cells maybe exist in placental HBV infection.