Study Of The Mechanism And Risk Factors Of Intrauterine Hepatitis B Virus Infection

Objective: 1. To investigate the risk factors of intrauterine hepatitis B virus (HBV) infection. 2. To study the mechanism of intrauterine HBV infection.Methods: 1. 174 HBsAg positive pregnant women were chosen. The peripheral blood hepatitis B virus markers (HBV-M) of their 176 newborns were detected within 24 hours after born, and then were followed up at 1 -month and 6-month. Based on the follow-up results, all cases were divided into either intrauterine infection group (group I) or non-intrauterine infection group (group Π). We analyzed the correlation between the clinical datas and intrauterine HBV infection, to screen the risk factors of intrauterine HBV infection.2. The HBsAg were detected by PV-9000 immunohistochemical staining in these placentas. By analyzing the correlated clinical datas, we tried to screen the high risk factors of placental HBsAg positive. The next step was to analyze the HBsAg positive rate and positive intensity of trophoblastic cells and villous vascular endothelial cells and the rate of HBsAg positive blood vessel in villus, and the relationship between the three factors and intrauterine HBV infection.3. 20 cases of villus/placentas from normal (HBsAg negative) pregnant women were collected in the first trimester (group A), the second trimester (group B) and at the term (group C), respectively. Another 20 cases of term placentas (group D) were selected randomly from 87 HBsAg positive placentas. (1) Quantitative analysis of placental FcγRIII in 4 groups was achieved by immunofluorescence assay and the confocal laser scanning technique. (2) The distribution of HBsAg and FcγRIII in placentas of group D were detected by double-label immunofluorescence assays and the confocal laser scanning technique.Results: 1. The follow-up results of infants showed that the HBsAg positive at 6-month could be used as a diagnostic criteria of intrauterine HBV infection. Group I had 9 cases, group Π had 167 cases, and the rate of intrauterine HBV infection was 5.1%. Maternal serum HBV-DNA positive, HBeAg positive and...