| Human group B rotavirus(GBRV) was first identified as causative agent of several large outbreak of severe gastroenteritis, predominantly in adults, by Hong Tao in China in 1983. In spite of serological evidences for the presence of group B rotavirus in many countries in the world, the virus has been detected only in China, India and Bangladesh. The objective of the research is to develop a rapid, sensitive and highly specific techonology for the detection of human group B rotavirus, which will be helpful for the study of GBRV including biological characteristic, epidemiological study, diagnosis as well as prevention and treatment.The first part of this thesis is a brief review on recent advance of GBRV.It focuses on the viral characteristic, epidemiology and diagnostic techonology .The nsp5 gene of GBRV was labelled with isotopic by polymerase chain reaction(PCR) .Its sensitivity and specificity was tested. The probe was also used to detect diarrhea specimen from Wuhan city. The results showed the sensitivity was 500pg with high specicity. 94 cases of diarrhea specimens in Wuhan were detected and one was positive. The positive sample was confirmed positive by RT-PCR and sequence determination. A pair of primer was designed based on the vp7 gene sequence of ADRV, a kind of group B rotavirus. The 5'-end of the primer was labelled with biotin. The probe was also prepared with PCR with a sensitivity of 50ng. Dig-labelled vp7 gene probe of GBRV was prepared with PCR.The primer was the same as the primer mentioned-above but it was not labeled at the 5'-end.The sensitivity was 10ng with high specicity. The nucleic acid hybridization assay described here could be used as a primary means for the detection of GBRV, and it should be useful in confirming the presence of GBRV in specimens identified by other techniques, in addition, it is supposed to play important role in the epidemiological study of GBRV.
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