The Effect And Mechanism Of RGD Peptide On Oral Squamous Cell Carcinoma

Purpose:1,Study on proliferation and apoptosis of Tb cells, to research the mechanism of RGDV on antitumor.2,Study on the growth and angiogenesis of hamster buccal-pouch carcinoma by synthetic peptide RGDV, to research the mechanism of RGDV on antitumor.Methods:1,Study on proliferation and apoptosis of Tb cells(1) The effect of RGDV on proliferation were investigated with MTT assay and colonogenic assay, and the expression of PCNA was measured with flow cytometry.(2) The effect of RGDV on apoptosis were measured with flow cytometry , FITC-Annexin V/PI , Hoechst/PI ,electron microscopeand DNA fragmentation assay. The expression of caspase-3 protein was observed by flowcytometry.2,Study on the growth and angiogenesis of hamster buccal-pouch carcinoma bysynthetic peptide RGDV:Establishing golden HBP Ca, we used the specimens of hamster 's oral buccal pouch. The specimens were embedded by paraffin traditionally.The expression of v-WF and Brdu were tested by using immunihistochhemical method. Statistically analyzed the result.Results:1,Study on proliferation and apoptosis of Tb cells(1) The effect of RGDV on proliferation: The results showed that the growth and proliferation rate of Tb cell decreased in vitro after the addition of RGDV, and the expression of PCNA decreased too.(2) The effect of RGDV on apoptosis: The results showed the apoptotic rates increased and the expressions of caspase-3 protein increased relatively in Tb cells which were treated with RGDV. The cell number in the S phase increased, it indicated the cancer cells were blocked in the S phase. The DNA Ladder and the photo of the Hoechst/PI assay also proved the effect of RGDV on the apoptosis. We use the immunofluorescent staining of caspase-3 for flow cytometric analysis, and the expression of the group treated with RGDV increased.2,Study on the growth and angiogenesis of hamster buccal-pouch carcinoma by synthetic peptide RGDV(1)Blocked RGDV the effects on the model of oral carcinoma :The RGDV group tumor volume is smaller than that of Con group, after measure analyze there was significant difference between RGDV group and Con group(P<0.05).(2)The expression results of v-WF after blocked by RGDV: v-WF were expressed in two groups, significant difference was found in RGDV group with MVD(P<0.05). (3)The expression results of Brdu after blocked by RGDV: Brdu were expressed in two groups, significant difference was found in RGDV group with PI(P<0.05).Conclusions:1,RGDV could regulate the proliferation of tumor cells to inhibit the cancergrowth.2,RGDV plays a crucial role in the development of tumor by apoptosis andcaspase-3 protein may be one of the important transductions in apoptosis of Tbcells.3,RGDV prevent the growth and proliferation of golden HBP Ca.4,RGDV play a role in tumor angiogenesis.