| ObejectiveHuman salivary gland adenocarcinma is one of the most common oral cavity maligant tumor which is serious to the life and health of human beings . The harm to human body still not only lies in the gram of growth of tumor cells lose contool but cause of the tumor cell clore to increase to abnormity hyperplasia. In the meantime , the protential of tumor cells growth , infiltration and metastasis are the main one of the reasons to cause human beings death by salivary gland adenocarcinoma .And vein neogenesis of tumor growth and metastasis is the precondition of tumor cells . Tumor can gein much more nourishment from host by tumor vein , and depend on it output a lot of malignant cells and led to tumor perpetual growth and metastasis Tumor's vein angiogenesis is associated with mary growth factors' instimulation for example EGF , BFGF , TGF , PDGF and VEGF , and so on . Among them , VEGF it is secreted by some tumor cells , and is combined receptor which is in blood vessel endothelium can induce tumor to continue to grow , is an important afferent and born factor , and many physiology and pathologic process relevant. And the experiment prove to have a VEGF the abnormality high expression inside most entity tumors , is the function is the most strong , one of the particularity growth factor of the tallest angiogenesis factors.EIF household member starts hat dependence the translation process of the protein, cell at normally under physiology cond ition, eIF household member low expression, be eIF household member's expressing excessively can cause the cell growth accelerate, and produce the change of appearance, even cause the cell cancer change.among them, the excesssive expression of eIF 4 Es can in aid of machine body to translation the start an upper stream choice for ordering, cause the increment that the VEGF mRNA translates.VEGF of the excesssive expression result in and organize the change of the form type, being advantageous to tumor cell occurrence, development, gradually with transfer.Elemene is our country withdraw from the root-stock of the ginger section plant curcuma armatica rhizome first of anti-cancer valid composition.the research discover elemene to the person salivary gland adenocarcinoma the cell SACC-83 stub represses a function to present time quantity dependence. for inquiry into Elemene to person salivary gland adenocarcinoma the cell SACC-83 stub repress a function, inquirying into Elemene to person salivary gland adenocarcinoma cell stub in true pit cell translation the start factor an eIF 4 Es and and tumor angiogenesis relevant of inside blood vessel endothelium factor VEGF proteinum expression of influence.this research draw up to use immunity cell the method examination of the chemistry a person ce salivary gland adenocarcinoma ll SACC-83 Es eIF-4s, VEGFproteinum level expression of change, study song eIF 4 Es, the expression of the VEGF and activity to repress a person the salivary gland adenocarcinoma to encroach upon of function, is Elemene and agreeable platinum of the clinical consociation use the medicine is applied in the salivary gland adenocarcinoma soon of the clinical treatment provide certain ories basis.Method:1 Use the cells SACC-83s of the Elemene processing of different density respectively, use agreeable the platinum add the cells SACC-83s of the Elemene processing at observing the appearance of cells SACC-83s not and meantime, applying immunity cell organization the chemistry SP method an examination an eIF 4 Eproteinum an expression a circumstance, using same method examination and tumor angiogenesis relevant of VEGF of expression.2 Adopt SPSS 11.0 softwares carry on covariance analysis. calculate data to use all few Xs S an expression, adopt a square bad the analysis learn to the experiment data covariance analysis, examination standard with a=0.05 for show examination level. Results:1 The Elemene of different density all can with the expression of the eIF 4 Es and the VEGF proteinum in the cells SACC-83s, Elemene with check against 22 compare, the difference all has covariance to learn meaning(P<0.05)2 The Elemene reduce eIF 4 Es, the expression of VEGF proteinum sometimes and measure dependence, along with the time extension, eIF 4 Es of the Elemene function, the VEGF proteinum expression descends one by one in order.3 The Elemene(60 ug/ml) amiable platinum(30 ug/ml) consociation's using a medicine can strengthen cells SACC-83s to repress.Conclusion:1 The cells SACC-83s eIF 4 Es with relevant tumor angiogenesis growth the proteinum high expression of the factor VEGF.2 Elemenes amiable platinum can repress the growth of cells SACC-83s, having time and density dependence. Elemene, the agreeable platinum consociation has higher growth to repress with the medicine rate, two medicines uniting an usage can raise medicine to repress tumor cell further.3 adoption immunity cell the method examination eIF 4 E of the chemistry, VEGF expression more blank and melting agent matched control are low, hinting the Elemene represses of the growth not only has something to do with proteinum synthesizing to repress, but also returns to have something to do with eIF household member causing a related proteinum expression to descend. |
PDF Full Text Request