| Objective To explore the relationship between microsatellite DNA polymorphism of human 1113-hydroxysteroid dehydrogenases type 1 gene and type 2 diabetes within Chinese population. Methods 150 patients with type 2 diabetes and 121 non-diabetic subjects were collected randomly among Han Chinese in Nanjing. Polymorphism of microsatellite marker within intron 4 of 1113-HSD1 gene was detected by performing polymerase chain reaction, polyacrylamide gel electrophoresis and silver staining to investigate the frequency distributions of 11β-HSD1 microsatellite (CA)n in two groups. Results In two groups, eight types of alleles were found with different CA-repeat numbers: 13, 14, 15, 16, 17, 18, 19 and 20. The prevalence of 11β-HSD gene allele frequencies in patients with type 2 diabetes was not significantly different from that in control subjects (χ~2=8.9944, P=0.253). However, the prevalence of (CA)IS allele gene frequencies in patients with type 2 diabetes was significantly different from that in control subjects (χ~2=4.990, P=0.025). Conclusion Polymorphism of microsatellite markers in 11β-HSD1 may be associated with type 2 diabetes in Hart Chinese. Objective To observe the expressions of 11β-hydroxysteroid dehydrogenase type 1 and type 2 in colorectal cancer and effects of glucocorticoid and glycyrrhetinic acid(GA) on colorectal cancer cell. Methods Human colorectal cancer cell line HCT-8 was cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, and passaged at intervals of 2～3 day. After HCT-8 cells were treated with glucocorticoid, cosubstrate, Glycyrrhetinic acid(GA) only or combined, the cell growth inhibitions were determined by MTT assay, the mRNA expressions of 11β-HSD1 and 11β-HSD2 were detected by reverse transcription polymerase chainreaction(RT-PCR), the protein expressions of 11β-HSD1 and 11β-HSD2 were detected by Western blot assay. Neoplastic and autologous non-neoplastic tissues were collected from 12 patients with colorectal cancer. The mRNA expressions of 11β-HSD 1 and 11β-HSD2 were detected by reverse transcription polymerase chainreaction(RT-PCR) and the protein expressions of 11β-HSD1 and 11β-HSD2 were detected by Western blot assay. The matched-pairs design t test was used to compare 11β-HSD mRNA and protein in the Neoplastic tissues with that in autologous non-neoplastic tissues. Results When treated with cortisol only, the cell growth inhibition rate was (40.87±1.47)%. Compared with the group of cortisol and NAD, there was significant difference (P＜0.01). When treated with GA, cortisol and NAD, the cell growth inhibition rate was (45.55±1.01)%. Compared with the other groups, there was significant difference (P＜0.05 or P＜0.01). 11β-HSD2 mRNA and protein were expressed in HCT-8 human colorectal cancer cell. Cortisol, GA only or combined could upregulate expressions of 11β-HSD2 mRNA and protein, and there was significant difference (P＜0.01) compared with the control group, 11β-HSD1 mRNA and protein were not expressed in HCT-8 colorectal cancer cell. Both isoforms of 11β-HSD were expressed in the colon adenocarcinoma. Compared with autologous non-neoplastic tissue, there was a significant decrease of 11β-HSD2 mRNA and protein in neoplastic tissue(P＜0.05), 11β-HSD1 protein and mRNA in neoplastic tissues were not significantly different from that in control tissues. Conclusion 11β-HSD2 may be associated with colorectal neoplastic transformation. GA can be used for antineoplaston. Glucocorticoid and glycyrrhetinic acid(GA) can induce the expression of 11β-HSD2 in colorectal cancer cell.
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