Expression And Clinical Significance Of Gene KLK11 In Ovarian Cancer

Ovarian cancer is one of the three most common malignancies Offemale reproductive system, mortality of ovarian cancer is the firstfemale reproductive system cancer mortality. The mechanisms of theovarian cancer is not clear. The Effective methods for early diagnosisand treatment are lack. The Human Kallikrein gene family contains15 genes. All genes share important similarities, including mapping at thesame chromosomal locus(19q 13.4). All members of the human kallikreinmultigene family encode for serine proteases that are found in diversetissues and biological fluids and involved in a variety of physiologicaland pathological processes. Borqono CA demonstrate that elevated serumlevels of HK11 were found in 70% of women with ovarian cancer,whichsuggest KLK11 expression may abnormal in the ovarian cancer.In this research, KLK11 mRNA expression was examined byRT-PCR. The expression and distribution of KLK11 in ovarian cancerwere detected by immunohistochemistry and image analytical technique.We analyzed the results combination with clinic pathological conference. The purpose of this study was to examine expression level ofthe human tissue kallikrein gene (KLK11) in ovarian cancer. Somolecule regulation mechanisms and experiment proof of ovarian cancercan be obtained. The aim of present study was to determine the potentialrole of KLK11 mRNA expression in ovarian cancer development andprogression. The result were analyzed in term of tumor type, clinicalstage and histological grade.Methods1.RT-PCRFor RT-PCR analysis, fresh surgical ovarian cancer tissue samplewere collected from 48 patients treated surgically, Extraction ofmRNA from ovarian tissue and cDNA synthesis were carried out.cDNA was synthesized by random hexamer priming. KLK11 mRNAexpression levels were determined by RT-PCR.Reaction product waspanned successfully using electrophoresis technique.2. Immunohistochemistry stainIt was operated by SP method, the monoclonal antibody of rat anti-KLK11 were used as first antibody with DAB colouration.3. Image analysisPositive staining of immunohistochemistry to ovarian cancer wastaken with quantitative analysis by Germany Leica RX 250 image analytical system. Relative areas and integral gray scale of positivestaining of KLK11 expression were measured in campus visualis.4. statistics analysisFor statistical analysis, spss11.0 software package was used toassess expression of KLK11 gene. Associations between clinicopathologicparameters, such as stage, grade, histotype, and residual tumor, andKLK11 expression were analyzed by the x~2 test,F test, and t test.Results1. RT-PCRHigh KLK11 mRNA expression remained significantly associatedwith clinicopathological features. The expression level of KLK11 genemRNA upload with the development of the clinical stage. Expression ofKLK11 mRNA levels showed significantly higher in patients with theadvanced stages (ⅢorⅣ) compared to the early stages (ⅠorⅡ)Expression of KLK11 mRNA levels showed significantly higher inpatients with the poor and moderate histological type than the goodhistological type. On the other hand, no significant association was foundin KLK11 expression grade of ovarian carcinoma2. Immunohistochemistry stainKLK11 protein was detected in the cytoplasm. The positive rate ofKLK11 protein showed significantly higher in patients with the advancedstages (ⅢorⅣ) compared to the early stages (ⅠorⅡ) The positive rate of KLK11 protein showed significantly higher in patients with thepoor and moderate histological type than the good histological type.There is no difference in Serous cystadenocarcinoma, Mucinouscystadenocarcinoma, Endometrial adenocarcinoma and Clear CellCarcinoma3. Image analytic resultRelative areas and integral gray scale of positive staining of HK11expression of the advanced stage and the poor or moderate cancerdifferentiation groups were higher than those of the early stage and thehigh differentiation groups, the difference has statistical significance.(P＜0.05)Conclusions1. KLK11 protein was detected in the cytoplasm. Expression of KLK11both on mRNA and protein levels showed significantly higher in patientswith the advanced stages (ⅢorⅣ) compared to the early stages (ⅠorⅡ).Expression of KLK11 both on mRNA and protein levels showedsignificantly higher in patients with the poor and moderate histologicaltype than the good histological type. On the other hand, no significantassociation was found in KLK11 expression grade of ovarian carcinoma2. The different expression of KLK11 might be related with themalignancy of ovarian carcinoma.KLK11 gene could play critical role as a prognostic marker in the future.