| The primary hepatic carcinoma(PHC) is one of the common carcinoma inChinese which occurring rate ascended recently. There are many similardescribes on symptoms about PHC in ancient records of traditional Chinesemedicine (TCM), such as "amass", "neoplasia" etc. The mechanism of this diseaseare seven emotion hurt, the etiological factor and pathogenesis cognitionstagnation of emotion, dampness clog caused by spleen difiency; intrude by sixevil pathogenesis and poison etc. PHC occurrence related with infecteddampness heat evil and poison, seven-emotion result in internal damage, Thecause have dampness, Heat, Phlegm, gloomy and stasis, etc. All of these pathogensmakes confusion of QI, blood, dampness, heat, stasis, poison and succeed PHC.There is still no standardized primary differentiation type and therapyprinciple about recognition on PHC. The treatment based on the differentialdiagnosis differential diagnosis in TCM has not yet been standardized atpresent, so it is great importance to make a united standard of differentialdiagnosis and do it objective, which will establish theoretical basis for theselection of treatment based on the differential diagnosis and improve theclinical effectiveness.Objective: To study the molecular mechanism on the changes of GGT,AMY activityand sugar chains of GGT,AMY of PHC cases with different TCM type.Methods: 1. to examAFP,GGT,AMY activity in sera of 96 cases (30 normal peoples,20 hepatitis patients, 15 hepatocirrhosis patients, 31 PHC patients). 2.Concanavalin A (ConA),Lens culinaris agglutinin (LCA),Phaseolus vulgarisleucoagglutinin (L-PHA),Peanut agglutinin (PNA),Pisum sativum agglutinin(PSA),wheat germ agglutinin (WGA)-binding ratios of serum GGT,AMY were determined in 96 cases(30 normal peoples, 20 hepatitis patients, 15hepatocirrhosis patients, 31PHC patients), then exam ONOO~-, MDA. 3. Analysisthe difference TCM type from PHC 31 cases, that is allocated three groups:QI and blood stasis,spleen deficiency with liver stagnation,liver and kidneyYin deficiency, then exam ConA,LCA,L-PHA,PNA,PSA,WGA-binding ratios ofserum GGT,AMY,serum GGT,AMY activity,ONOO~-,MDA. 4. the relationshipbetween ConA,LCA,L-PHA,PNA,PSA,WGA-binding ratios of serum GGT,AMY andMDA were determined.Results: 1. The volume of AFP,AMY activity in PHC cases is higher than normalgroup, but have no significant differenence, as compared to hepatocirrhosisand hepatitis groups (P＞0.05), meanwhile GGT activity in PHC cases is higherthan hepatocirrhosis and hepatitis groups (P＜0.01). 2. L-PHA,WGA-bindingratios of serum GGT in PHC cases were significantly higher than other threegroups(hepatocirrhosis, hepatitis and normal groups, P＜0.01); LCA,PSA-binding ratios of serum GGT in PHC and hepatocirrhosis cases weresignificantly higher than hepatitis groups and normal groups (P＜0.05). ConA,LCA,L-PHA,PNA,PSA-binding ratios of serum AMY in PHC cases were significantlyhigher than hepatitis groups and normal groups (P＜0.05), there are nosignificant difference, as compared to hepatocirrhosis groups (P＞0.05).3. Serum MDA in PHC and hepatocirrhosis group were significantly higher thanhepatitis and normal group (P＜0.05), while the level of ONOO~-, there areno significant difference between every group. 4. About the level of AFP,GGT,AMY,ONOO~- and MDA, there are no significant difference between three TCM typefrom PHC cases (QI and blood stasis, spleen deficiency with liver stagnationand liver and kidney Yin deficiency) (p＞0.05). LCA,PNA,PSA,WGA -bindingratios of serum GGT in spleen deficiency with liver stagnation group weresignificantly higher than liver and kidney Yin deficiency group(P＜0.05);LCA,PSA-binding ratios of serum AMY in spleen deficiency with liverstagnation group were significantly higher than liver and kidney Yindeficiency group(P＜0.05); 5. A positive correlation was found between L-PHA,PSA,WGA-binding ratios of serum GGT and MDA. A positive correlation was foundbetween LCA,PNA,PSA-binding ratios of serum AMY and MDA.Conclusion: As the proceeding of liver disease, in the molecule of serum GGTof PHC patients, triantennary and tetraantennary complex-type sugar chainsincrease, all of them improved the development of PHC, GGT containing multiantennary complex type sugar chain is correlative with PHC, inaddition, core-fucosylated of high-mannose-type and hybrid-type sugar chainsincreased; in the molecule of AMY of PHC patients, core-fucosylated ofhigh-mannose-type and hybrid-type sugar chains increased, terminal sialicacid and fucose on the sugar chain decrease, D-Gal on the sugar chain exposedmore, in addition, triantennary and tetraantennary complex-type sugar chainsincrease, this may be causes by increasing free radical damage amylase sugarchains, ln our research, there are significant difference in GGT,AMY sugarchains of three TCM type from PHC cases (QI and blood stasis, spleen deficiencywith liver stagnation and liver and kidney Yin deficiency), in spleendeficiency with liver stagnation group, core-fucosylated of high-mannose-typeand hybrid-type sugar chains of GGT,AMY increased. Taken together, we suggestthat there are the changes of GGT,AMY sugar chains relating to TCM type.
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