The Primary Study For The Target Region Drawing Of NSCLC By Detection Of P16 Gene Methylation

Objective: To explore the value of designing the target region by using the difference ofp16 gene Methylation between the tissues of Non-small cell lung cancer and the tissuesadjacent the lung cancer.Methods: A total of 33 cases of pathological tissues were included in this study. Allspecimens were obtained from patients who underwent surgical resection, among them,29 cases were Non-small cell lung cancer, and 4 cases were benign lung diseases. Aftergeneral dealing with the samples, DNA extracting and Methylation-specific PCRamplification, the PCR products were dyeing with ethidium bromide staining andobserved under ultraviolet lamp.Result: Among 29 Non-small cell lung cancer tissues, abnormal methylation was found inpromoter area of p16 gene in 12 cases (41.4%). Among them: 6 cases wereadenocarcinoma, 3 cases were squamous carcinoma, 2 cases were bronchioloalveolarcell carcinoma, 1 cases were adenosquamous carcinoma. The abnormal methylation werenot found in both the benign lung diseases and the tissues that adjacent lung cancer.Methylation of p16 was more frequent in Non-small cell lung cancer cases than in bothbenign cases and the tissues that adjacent the lung cancer(P＜0.05). There were no tumorcells in microscope in the tissues that adjacent the lung cancer. Methylation of p16 wasprobably higher in poor differentiated pathological types (P=0.05). Methylation of p16was no difference between the different pathological types and different stage(P＞0.05).Conclusion: Methylation-specific PCR can be used to guide designing the NSCLC targetregion in precision radiotherapy.