| Objects To extractβ-c from Dunaliella salina.; To establish the methods of determining the content ofβ-carotenes and 9-cis-β-carotene; To preparateβ-c from Dunaliella salina. submicron emulsion and study its protective effects on thymocytes in Kunming mice damaged by 60Coγ-ray(3 Gy). Methons Theβ-C was extracted from Dunaliella salina. by airtight extract cask; The method of gradient elution high performance liquid chromatography (HPLC) was established to determine the content ofβ-carotenes and 9-cis-β-carotene; The orthogonal design was adopted to optimize the preparation procedure and prescription; The irradiation damage models of thymocytes were established by 60Coγ-ray(3 Gy). Cells were randomly divided into six groups: control, 60Co model, 60Co＋0.64μg·mL-1β-C,60Co＋0.32μg·mL-1β-C,60Co＋0.16μg·mL-1β-C and 60Co＋0.1mg·mL-1VitE groups. The protective effect ofβ-C on thymocytes irradiated by 60Co was studied with the MTT chrom atometry and Brdu; Apoptosis was observed by agarose gel electrophoresis; The expressions of p38 proteins were examined by western blot. Results The content ofβ-c and 9-cis-β-c in extract was 226.77mg·g-1 and 84.60mg·g-1 respectively; The microphotograph with electron microscope showed that most of emulsion drops were round in shape. The results of accelerating and long-term stability tests indicated the physical and chemical stability of submicron emulsion were fine. Theβ-C from Dunaltella salina could improve cytoactive and cell proliferation significantly, decease DNA ladder and inhibit the expression of p38. Conclusions The extract technology was efficient, practical, low-cost and low-pollution; The method of HPLC was high resolving and reproducibility; The prescription and preparation procedure were reasonable and its properties were stable; Theβ-C from Dunaliella salina, had protective effects and the antiapoptotic effects on damages of thymocytes caused by the 60Co irradiation with direct correlation during 0.16～0.64,μg/mL.
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