Studies On The Active Constituent In Pedicle Leaf Of Lamiophlomisrotata

The plant Lamiophlomisrotata (Benth.)Kudois used in abirritation and hemostasia. Iridoids is Lamiophlomisrotata(Benth.)Kudo. Yhe application of reactive component like Iridoids in Lamiophlomisrotata(Benth.)Kudoinclud the pharmacdogical action on abirritation and hemostasia. Moreover, the pharmacopoeias of 2005 edition lacks the quantitative investigation, So we studies its steroidal saponins ingredient and seek the monomer active constituent. It is significant to find and establish the effective content determination method for quality control of the preparation and crude drugs.Screening test of Analgesic and hemostatic portion. The 70ï¼…ethanol extract in stem and leaves of Lamiophlomis rotata (Benth.)Kudowas isolated by polyamide into two portion, the portion eluted by water has the better Analgesic and hemostatic effect.So using AB-8 macroporous resin,we purified the above portion and obtained five ethanolic elutions, the 30% ethanolic elution show strong Analgesic and hemostatic action.We studied on Iridoids which is the active part of Lamiophlomisrotata (Benth.) Kudo. The method of silica column chromatography, thin layer chromatography and gel column chromatography was used to isolate the chemical constinents. Their structures were identified by physicochemical properties and spectra analysis (1H-NMR and 13C-NMR). Eight compounds were isolated and their structures were identified as -sitosterol, 8-0-acetyl-shanzhiside, methyl ester, shanzhiside, 6-0-acetyl- shanzhiside methyl ester, methyl ester, sesamoside, dehydropentste-moside, 7- deoxyloganic acid is known first finded compound in Lamiophlomisrotata (Benth.).Pharmacological screening test of compound 6. Compound 6 was evaluated by the mouse stretching-body method, tail-cutting method and auricular-swelling method, compound 7-deoxyloganic acid can be affirmed as the active compound of hamiophlomis rotata (Benth.) Kudo.Studies on extraction and purification process, the best extraction was established by orthogonal test. solvent: 60% ethanol, soak:36 hours, elution volume:f5 times of materials .Pufification process:the ethanolic extract should be isolated by polyamide. Then isolate the water elution with XDA-7 macroporous resin and collect the 20% ethanolic extract.