Epileptic Model Induced By Brucine And TDAG51 Expression In The Model

Objective: To identify the possibility and method of brucine in the establishment of brucine induced epilepsy model in rats according the fact that Nux Vomica could induced seizers in human.At the base of the establishment of epilepsy animal model induced by brucine, We want to investigate the change of TDAG51 (T cell death-associated gene 51, TDAG51) protein which is releated with apoptosis in cerebral tissues. Methods: 43 rats were randomly diveded into experimental group,negative model group and control group. We observed the rat's behavioral changes by single intraperitoneal injection of brucine and repeated intraperitoneal injection of brucine at different dosages in experimental group.We need dissolve brucine with absolute alcohol,and then add normal sodium before injection.(absolute alcohol: normal sodium 13:100)The negative model group rats were injected the same volume of composition with absolute alcohol and normal sodium. ( absolute alcohol: normal sodium 13:100)The control group rats were injected the same volume of normal sodium. The epileptic and survivaled rats were diveded into model group. The cerebral cortex and hippocampus of model group rats were studied by using immunohistochemistry for MAP2 (Microtubule associated protein2, MAP2) expression on day 7. Control brain tissues were used for comparison. 22 rats were randomly diveded into experimental group, negative model group and control group.we copied the epilepsy model induced by brucine by repeated intraperitoneal injection of brucine at 91mg/kg. The epileptic and survivaled rats were diveded into model group. Immunohistochemistry method was also used to measure the expression of TDAG51 protein on 2 day. Results: In the groups injected with 91 mg / kg -110 mg / kg once, the seizures could be observed.when we increased the dosage of injection,we observed more seizures.But the mortality rate was high. In contrast,the ratio of death decreased in repeated intraperitoneal injection groups injected the same dosage.There is no significant difference on achivement ratio of model between 91mg/kg and 100mg/kg ,in which the brucine was injected repeatly(P>0.05).The immunohistochemistry data showed that the expression of MAP2 in model group was significant higher as compared with the control groups(P<0.05).The expression of TDAG51 protein was detected in the cytolymph of nerve cells. Compared with control groups,the expression of TDAG51 protein was significant higher(P<0.05). Conclusion: (l)our study provided an acute and systermic epilepsy model induced by brucine, in which the suitable dosage is 91 mg / kg -110 mg / kg and the suitable method is repeated intraperitoneal injection .The interval is 30 minutes. (2)Expression of TDAG51 was higher in model group,which suggest that the expression of TDAG51 may play an important role in apoptotic process induced by seizure.