Effect Of SVF/ADSCs On Growth Of Rat Dorsal Root Ganglia In Vitro

Background:The regenerationof peripheral nerve repair has not been solved yet.In recent years,both of domestic and foreign researchers conducted intensive studies on the neural tissue engineering whitch was thereplacement of autologous nerve garft substitute.Stromal vascular fraction(SVF) was a heterogeneous collection of cells such as the prsadipocytes,bone marrow derived mesenchymal stromal cells,endothelial progenitor cells,T lymphocytes,B lymphocytes,mast cells and macrophages.The SVF had a rich source and the advantages of easily expandes in culture and the excellent differentiation and proliferation ability.In the meanwhile it can promote vascularization and reduce the risk of infection.Objective:The SVF contains a large number of stem cells and stromal cells,it was used as the treatment in many different fields.But there was no article showed that the SVF can promote the regeneration of nerve injury.So this article mean to investigate the effect of the SVF and ADSCs on regeneration of the nerve injury.Methods:Four male Sprague Dawley(SD) rats(weighing 200g) were selected to isolate SVF which was collected from adipose tissue on both sides of the groin. The ADSCs was cultured and amplificated by SVF in vitro.According to different variables,the experiment was divided into 3 groups,group A(1×106 cells/20 ul SVF),group B(1×106 cells/20 ul ADSCs) andgroup C(Blank control).Each group were co-cultured with rat DRG.The axon length of DRG,Schwann cell migration distance,and axon area index werequantitatively evaluated by morphology,neurofilament 200 immunofluorescence staining after cultured for 48 hours.Results:The DRG cultured for 48 hours in vitro,each groups been compared in three different ways,such as axon length,schwann cell migration distance and axon area index.The results showed that the axon length and schwann cell migration distance in group A was significantly greater than group B(P<0.05).The axon length and schwann cell migration distance in groups A and B was significantly greater than that in group C(P<0.05).The axon area index in groups A and B was significantly greater than that in group C(P<0.05).Conclusion:1.Both SVF and ADSCs ingredients can promote DRG neurite outgrowth and migration of Schwann cells.2. The SVF was more effective than ADSCs in promoting dorsal root ganglia neurite outgrowth.3.The SVF can be used in regeneration of nerve injury.4.The using of fibrin glue-coated can actually simplified the processes of making neonatal rat DRG model.