Investigating Gene Expression Profiling Of Psoriasis Vulgaris

Psoriasis is a frequent and chronic skin disease which attacks recurrently. Thecause and the pathogenesis of this disease haven't been completely bright yet. A moredefinite understanding is a interactive result of the genetic factor and the environmen-tal factor affecting mutually. Along with the human genome project rapidly develop-ing and the molecular genetics technology unceasingly emerging, it has provided thesupport in technics and methodology for the localization and the recognition of thegenes related to psoriasis. We investigated the gene expression profiling of peripheryblood from psoriasis vulgaris patients in order to discuss the expression and thefunction of the related gene groups of psoriasis, further confirm the change of genegroups level during the process of psoriasis, seek predisposing gene groups andprovide the new way for the prevention and the treatment of psoriasis.We choosed 4 samples of psoriasis vulgaris and 1 example normal person as thecomparison, collected the periphery blood and separated lymphomonocyte. Then weextracted total RNA and carried on the quality assessment. High-quality total RNAwas been successfully isolated. Then these total RNA were purified and reverselytranscripted to single-stranded and double-stranded cDNA. After being purified andan in vitro transcription reaction, the cDNA was then done to produce biotin-labeledcRNA. Then the obtained "targets" were hybridized to 5 Affemetrix HU-U133 Plus2.0 gene chips(represented 38,500 definite human genes). After being stained andstrictly washed, the hybridized probe arrays were scanned by the Affymetrix Genearray Scanner 3000 7G. Specific experimental information was defined and different-ially expressed genes of fold changes(≥2.0) were picked up by using GeneChipOperating Software(GCOS). The results showed as the following:①By applying this chip assay to the sick and the normal periphery blood, we found that the number ofdifferentially expressed genes were 505,489,1657 and 1788 respectively betweeneach patient of psoriasis vulgaris and the normal person. The majority of these geneswere upregulated and the minority of them are downregulated. We also identified atotal of 50 genes that common differed in expression in the patients versus normalcontrol. Among them 17 genes were upregulated (accounted for 34.0%) and 33 geneswere downregulated (accounted for 66.0%). These differentially expressed genes arealways involved in many kinds of vital activity including the transportation, thecellmetabolism and signal transduction, the growth, the oncogenes, the proteintranslation and synthesis, the cell cycle, the cytoskeleton composition and movement,the immunity, the DNA combination and transcription, the stress reaction, theapoptosis and cell acceptor, etc.②A part of genes haven't reported before in countryand abroad. These genes are signal-transduction-related genes include CDC42effector protein 3(CDC42EP3),purinergic receptor P2Y13(P2RY13),serine/threoninekinase 17b(STK17B),synaptotagmin-like 3(SYTL3),phosphodiesterase 4B (PDE4B)and regulator of G-protein signalling 1(RGS1), etc; DNA combination andtranscription related genes and transcription factors include cAMP responsive elementmodulator (CREM), NF-κB inhibitorζ(NFKBIZ),likely homolog of yeast SEN54(SEN54L),and v-maf musculoaponeurotic fibrosarcoma oncogene homolog F(avian)(MAFF), etc; protein translation and synthesis related genes such as prostaglandin-endoperoxide synthase (PTGS); apoptosis and stress reaction protein related genessuch as cold autoinflammatory syndrome 1 (CIAS1) and immediate early response 3(IER3); cell-acceptor-related genes include nuclear receptor subfamily 4, group A,member 2(NR4A2),LR8 protein (LR8) and gamma-aminobutyric acid receptor-associated protein like 1 (GABARAPL1), etc; metabolism-related genes such asdual specificity phosphatase 2 (DUSP2) and 6-phosphofructo-2-kinase / fructose-2,6-biphosphatase 3 (PFKFB3), etc; growth-related genes such as amphiregulin (AREG)and epiregulin (EREG), etc; cell cycle protein related genes such as putative lympho- cyte G0/G1 switch gene (GOS2) and so on.；③Immunity-related genes, such as IL-8,IL-1 beta,TNF, were downregulated in untreated patients versus normal control. Thisresult is opposite with the result of single gene in former literature reports.In summary, psoriasis is a disease of multifactorial inheritance. It arises is notthe result of single gene mutation, but is the result which is gene groups composed bymany genes affect together. Each gene action is quite slight, each other does not havethe dominant and the recessive relations. But many genes and gene groups haveformed the complex network regulatory system and have the interaction betweenthem. They have participated in the pathogenesis process of psoriasis together. Partialgenes related to psoriasis are discovered for the first time and the findings of a part ofgenes are opposite with the results of single gene in former literature reports.