Protective Effects Of Oyster Extraction On Chemical Liver Injury

The liver which is explained as"the biggest chemical plant in body"is the largest substantial gland and central station of metabolism in vivo. Being the important detoxication organ, the liver is the important target of drugs, xenobiotics and oxidative toxicant. Liver injury is the starting link and common approach of serious liver disease occurrence, development and finally moving towards the liver function failure. The liver injury being caused by various harmful factors were manifested as hepatic necrosis, fatty liver, cholestasis, liver fibrosis, liver cirrhosis and liver cancer and so on. Oyster is one kind of famous marine seafood, has been well characterized to possess widely pharmacological activities. In order to clarify the involved details, we investigate the effects of oyster extraction on chemical liver injury in vitro and in vivo in the present study.Firstly, the effects of compound isolated from the oyster by oral administration on carbon tetrachloride-induced liver injury in mice were examined. Then, alanine minotransferase (ALT), asparatate aminotransferase (AST), lactic dehydrogenase (LDH), nitrogen monoxidum (NO) and nitric oxide synthase (NOS) in blood serum, malondisldehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH), glutathione peroxidase (GSH-Px) in liver tissue were performed in the following in vivo experiments. Subsequently, the hematoxylin eosin (HE) pathological section and fluorescence staining for apoptosis assay were performed to clarify the possible mechanisms of 35# compound isolated from the oyster on chemical liver injury.Taken together , the results indicated: (1) 35# oyster extraction (50mg/Kg) had significantly suppressed the elevation of ALT, AST and LDH activities in blood serum (P<0.01). (2) 35# oyster extraction (50mg/Kg) had significantly suppressed the elevation of MDA and GSH contents in liver tissue, inhibited the reduction of SOD, CAT and GSH-Px activities in liver tissues (P<0.01). (3) 35# oyster extraction (50mg/Kg) had significantly enhanced the NO content and NOS activity (P<0.01). (4) 35# oyster extraction (25μg/ml) had enhanced the survival rate in L02 induced by CCl4 and H2O2 (P<0.05), significantly suppressed the elevation of ALT and AST (P<0.01). (5) 35# oyster extraction (25μg/ml) had significantly inhibited the L02 and CCC-HEL-1 cell apoptosis induced by H2O2.Conclusion: 35# oyster extraction had obviously protective effects on acute liver injury induced by CCl4 in mice and acute liver cell injury induced by hydrogen peroxide in vitro, which might be related the effect of anti-oxidation, protection of liver cell membrane and inhibition of liver cell apoptosis.