A Experimental Study With Fluid-percussion Model Of Traumatic Brain Injury At High Altitude In Rats

Object:To establish a experimental platform of a traumatic brain injury and improve the quality of the experiment,we try to develop a fluid-percussion traumatic instrument and optimize experiment route. To establish a fluid-percussion traumatic brain injury model of quantizing and grading, and give a experimental study at high altitude with the model.Methods:1.Refered the basic principle of fluid-percussion transmission and accelerational brain injury. Designed and made the fluid-percussion equipment which can adjust precisely and export attack pressure by different demand. 2.Established the fluid-percussion traumatic brain injury model with the fluid-percussion equipment: 32 adult SD rats were divided into two groups:control group (n =8) and injury group (n=24). The injury group was also divided into 3 sub-groups, there were 8 rats in every sub-groups. The 3 sub-groups were received 0.5-1.0atm,1.5-2.0atm,2.5-3.0atm pressure respectively , then their behaviors and pathology were evaluated. 3.Observate of brain injury in high altitude and low altitude with fluid-percussion model:60 adult SD rats were divided into two groups:low altitude experiment group (n =30) and high altitude experiment group (n=30). Every experiment were divided into two groups:control group (n =6) and injury group (n=24).and every injury group was also divided into 4 sub-groups by injury time, there were 6 rats in every sub-groups. The standard operating procedure was used in both experimental group and control group. For the control group we did not made fluid-percussion impact injury, the experiment made the rats into moderate traumatic brain injury models with 1.33-1.69atm pressure. We observed and compared the change of neuromotor and pathology injury in high altitude and low altitude,and understanded the characteristics of microcirculation in brain injury.Results:In our experiment we have designed and made the fluid-percussion equipment which can adjust precisely and export attack pressure by different demand.This instrument can give the animals precisely attack pressure in the angle state of balance weight. Neuromotor dysfunction, pathology injury ,water content of brain tissue and vWF in the blood-serum were graduately aggravated after increasing fluid-percussion impact. They were significantly increased comparing to low altitude experiment group in high altitude experiment group.Conclusion:The fluid-percussion traumatic equipment has high precision, high stability,and better repeat in operating.With the above animal fluid-percussion impact injury system we can repeat many clinic hackneyed traumatic brain injury models,and the traumatic brain injury model in high altitude has made a platform of animal experiment for more research of traumatic brain injury.