Expression And Significance Of ERα,ERβ And CyclinD1 In Bladder Transitional Cell Carcinoma

Objective :To study the expression and significance of ERα,ERβand CyclinD1 in bladder transitional cell carcinoma and to evaluate their significance in the generation,progression and prognosis of the bladder cancer.Methods: Expression of ERα,ERβand CyclinD1 were investigated by immunohistochemistry technique with 62 cases of bladder transitional cell carcinoma and 12 normal bladder cases.Results: There was no case expression of ERαin normal bladder tissue ,only 3 human bladder cancer weakly expressed ERαin 62 tumors. There was litter difference between them (p>0.05). The positive rate of ERβin BTCC ( 43.5%) was significantly higher than in normal bladder tissue(8.3%). No association was found between ERβpositive rate and patients'age , sex , tumor multiplicity and metastasis of lymph node .The positive rates of ERβin various pathological type and clinical stage were as follows : 30% of G1, 40% of G2, 64.7% ofG3,32.4% of Ta-T1 and 60% of T2-T4.There was significant difference of the positive rate and expression intensity between G1,G2 and G3(P<0.05) ,so was it between Ta-T1 and T2-T4 (P<0.05). The expression of CyclinD1 in the normal bladder tissue was negative. The positive rate of CyclinD1 in BTCC (53.2%) was significantly higher than that in the normal bladder tissue (P<0.01). No association was found between CyclinD1 positive rate and patients' age ,sex and metastasis of lymph node ,but the association was found between the tumor multiplicity and the expression of CyclinD1 (p<0.05 ). The positive rates of CyclinD1 in various pathological type and clinical stage were as follows : 85% of G1, 52% of G2, 17.6% of G3, 64.9% of Ta-T1 and 36% of T2-T4. There was significant difference of the positive rate and expression intensity either among G1, G2, G3 (P<0.05) and so was it between Ta-T1 and T2-T4 (P<0.01). There was a slightly inverse correlation between the expression of ERβand CyclinD1 (r=-0.285, P<0.05). The overall recurrence rate was 43.5% in 62 cases of bladder transitional cell carcinoma . The expression of ERβor CyclinD1 was a strongly relationship with no recurrence of survival rate and intervals times (p<0.05).Conclusions: We find the expression of ERαin the normal bladder tissue was almost negative . It was found that the rate of positive expression of ERβin the BTCC was positively correlated with the tumor grade and clinical, but the rate of positive expression of CyclinD1 in the primary BTCC was a negatively correlated with the tumor grade and clinical. Analysis of ERβand cyclinD1 expression in BTCC was useful for us to estimate the malignance and prognosis.