Hypomagnesemia, Endothelial Nitric Oxide Synthase Polymorphism, And Risk Of Coronary Artery Disease

Objective To investigate the association of the endothelial nitric oxide synthase(eNOS) gene polymorphism and serum magnesium ions concentration with coronary heart disease (CHD).Methods For 120 patients with CHD and 180 unrelated health individuals, the G894T mutation at exon 7 of the eNOS gene was studied by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)analysis and serum magnesium ion concentration was tested by automatic biochemistry analyzer.Results (1)The frequencies of eNOS GG, GT and TT genotypes were 0.889,0.089 and 0.022 respectively;G and T allele frequencies were 0.933 and 0.067 respectively.(2)GT+TT genotype frequencies in CHD and control group were 0.333 and 0.111 respectively(P<0.05);frequencies of T allele in CHD and control group were 0.192 and 0.067 respectively(P<0.05).(3)G894T mutation was not related to the number of affected vessels in CHD patients. (4) The serum Mg2+concentration in patients with eNOS 894TT genotype were lower than that in patients with eNOS 894GG genotype.(P <0.05) . (5) The serum magnesium ion concentration in patients with eNOS 894TT genotype was lower than that in patients or controls with eNOS 894GG genotype.(P <0.05) .(5)The coexistence of hypomagnesemia and G894T mutation are compounded with more affected vessels in CHD patients(P<0.05). Conclusion The G894T polymorphism in eNOS gene is associated with the presence of CHD, and in conjunction with hypomagnesemia, increased the risk of CHD severity.