The Relation Of Interleukin-1B Gene Polymorphism And Helicobacter Pylori Infection To Gastric Cancer

Objective To investigate the association of the interleukin-1B (IL-1B) gene polymorphism with gastric cancer in Hengyang Chinese population,and to study which type may be the sensitive genotype to gastric cancer in patients with helicobacter pylori (HP)infection.Materials and Methods For 52 patients with gastric cancer and 55 patients with chronic gastritis,the polymorphism of the IL-1B gene was studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, C/C,T/T genotype were sequenced.The infections of HP were determined by RUT and PCR assay .Comparison of genotype frequencies between cases and controls was assessed byχ~2 statistics.The frequencies of genotype on the risk of gastric cancer was expressed as odds ratios(OR) with 95% confidence interval(CI).Results The frequencies of IL-1B-31T, IL-1B-511T, IL-1B-31T/T and IL-1B-511T/T genotype were higher in gastric cancer group than in chronic gastritis group (P < 0.05), with odds ratios(ORs) of 1.97 (95%CI=1.15-3.59),2.52(95%CI=1.45-4.39),2.71(95%CI=1.10-6.66)and 3.33 (95%CI=1.14-9.73), respectively.There were no significant differences among HP infection rate to gastric cancer group , chronic gastritis group and pathology diagnosis. The frequencies of IL-1B-511T/T genotype were higher in gastric cancer group than in chronic gastritis group (P<0.05)with HP infection. The frequencies of IL-1B-511C/T genotype were higher in intestinal type gastric cancer than diffuse type gastric cancer (P<0.05).Conclutions In the HengYang Chinese population , the IL-1B-31T/T and IL-1B-511T/T genotypes may be associated with the high risk of gastric cancer, IL-1B-511 gene polymorphism may be associated with intestinal type gastric cancer , IL-1B-511T/T may be the sensitive genotype of gastric cancer in patients with HP infection.