Arrest Effect Of Diallyl Disulfide On Cell Cycle In Human Nasopharyngeal Carcinoma Cell Line CNE2

Objective: To investigate the arrest effect of diallyl disulfide (DADS) in the cell cycle of human nasopharyngeal carcinoma CNE2 cell line and its molecular mechanism.Methods:The growth inhibition effect of DADS on CNE2 cell line was measured by MTT assay and cell counting. Light microscope was employed to observe the morphlogical change after DADS treatment. Phase distribution of cell cycle was analyzed by flow cytometry. Expression of H3, H4 and P21WAF1 were determined by western blotting analysis.Results: The MTT assay showed that DADS inhibited growth of CNE2 cells significantly and exhibited a dose dependent modal. Adding 90μmol/L,140μmol/L,240μmol/L and 400μmol/L DADS for 48 hours suppressed CNE2 growth by 3.33%,12.90%,28.38%,56.90%, respectively. Cell counting showed that average doubling time retarded from 24.51h in normal cultured CNE2 cells to 93.10h in 400μmol/L DADS experimental CNE2 cells (P<0.05). Light microscope examination indicated that heteromorphism and karyoplasmic ratio were decreased when treated with 90μmol/L,140μmol/L,240μmol/L and 400μmol/L DADS for 48 hours in a dose and time dependent. Flow cytometry analysis revealed that treating CNE2 cells with increasing quantities of DADS increased the percentage of cells in the G0/G1 phase. By western blot, the acetylation level of histone H3 in CNE2 cell line was elevated after treated by DADS, and the maximum effect was higher 42% than untreated cells at 48h. At the same time,the expression of p21WAF1 also increased in a dose dependent. But the acetylation level of histone H4 did not changed.Conclusion:1. DADS had a role in inhibiting the proliferation of CNE2 cell line in a dose dependent.2. The antiproliferation property of diallyl disulfide (DADS) in cultured human nasopharyngeal carcinoma CNE2 cell line relates to its ability to arrest G0/G1 through over-expression of P21WAF1.3. DADS might be a potential deacetylation drug.