Perfusion Of Interleukin-1 Receptor Antagonist Attenuates Spinal Cord Injury After Aortic Cross Clamping

Objective: To investigate the impact of IL-1 ra, a anti-inflammatorycytokine, on cytokine microenvironment and spinal cord injury afterexperimental sub-renal aortic cross clamping.Methods: Rabbits were divided into two groups: control group, underwent aortic cross clamping with PBS perfusion; IL-1 ra group, underwentaortic cross clamping with IL-1ra (5μg/kg)administration through aorta.immediately and through 48 hours after the operation. Cell damage wasanalyzed by observing the function of the lower limbs and counting thenumber of motor neurons. Semi-quantitative RT—PCR was employed toexamine Tumor necrosis factor-α(TNF-α)and Interleukin—10 (IL—10)mRNA expressionResults: IL-1 ra attenuates the functional deficits of rabbit hind limbs andpreserved the number of motor neurons after ischemia compared withcontrol group. At 4 h and 8h post-operatively, the mRNA levels of TNF-αin the spinal cord of the IL-1 ra group were decreased, whereas IL—10levels increased markedly. These changes made the ratio of TNF-α/IL-10 dropped significantly Conclusion: IL-1 ra ameliorates the overwhelming inflammatoryresponse via a mechanism of suppressing the ratio of pro-/anti-inflammatory cytokine expression, which reflects potential clinicalimplications of use of anti-inflammatory agents in thoracoabdominalaortic surgery...